Mouse Tyrosine-protein kinase receptor UFO(AXL) ELISA Kit

货号: IPD-EL002476MO

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
小鼠酪氨酸蛋白激酶受体UFO(AXL)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆.样本中的AXL含量。AXL 作为重要靶点，属于受体酪氨酸激酶家族成员。它参与细胞增殖、迁移等多个生理过程，在多种肿瘤中呈现高表达，推动肿瘤生长、转移与耐药。目前对其研究聚焦于揭示激活机制，以开发有效靶向治疗药物对抗癌症。试剂盒检测范围为31.25 pg/ml-2000 pg/ml，灵敏度为7.81 pg/ml。例如探究肿瘤模型中AXL表达与微环境互作、评估炎症条件下受体激活状态，或筛选靶向AXL通路的候选化合物。可为细胞信号转导、疾病机制及药物干预研究提供高特异性定量支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Axl ELISA Kit; Ark ELISA Kit; Ufo ELISA Kit; Tyrosine-protein kinase receptor UFO ELISA Kit; EC 2.7.10.1 ELISA Kit; Adhesion-related kinase ELISA Kit
缩写：
AXL
Uniprot No.：
Q00993
种属：
Mus musculus (Mouse)
样本类型：
serum, plasma, tissue homogenates.
检测范围：
31.25 pg/ml-2000 pg/ml
灵敏度：
7.81 pg/ml
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Cancer
测定原理：
quantitative
测定方法：
Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(pg/ml)249.462250.052250.774249.134251.891248.806SD0.0240.0260.0290.0340.0320.035CV(%)4.4684.8335.3796.3365.9176.529

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse AXL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	106
	Range %	98-111
1:2	Average %	100
	Range %	95-108
1:4	Average %	96
	Range %	88-101
1:8	Average %	94
	Range %	85-98

回收率：

The recovery of mouse AXL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	101	95-110
EDTA plasma (n=4)	97	91-102

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.










pg/ml	OD1	OD2	Average	Corrected
2000	2.162	2.062	2.112	1.989
1000	1.339	1.444	1.391	1.268
500	0.882	0.905	0.894	0.771
250	0.537	0.515	0.526	0.403
125	0.322	0.306	0.314	0.191
62.5	0.220	0.231	0.225	0.102
31.25	0.156	0.154	0.155	0.032
0	0.125	0.121	0.123