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Rat apolipoprotein A1 (Apo-A1) ELISA Kit

  • 中文名: 大鼠载脂蛋白A1(Apo-A1) ELISA Kit
货号: IPD-E08105r
Price: ¥2500
数量:
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产品详情

  • 产品描述:
    大鼠载脂蛋白A1(Apo-A1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的APOA1含量。APOA1是重要靶点。它是高密度脂蛋白(HDL)的主要载脂蛋白,与胆固醇逆转运相关,有助于将外周组织胆固醇转运回肝脏代谢。研究发现其在动脉粥样硬化等疾病进程中发挥关键作用,通过提高其水平或活性有望改善脂质代谢、防治相关疾病。试剂盒检测范围为0.14 ng/ml-100 ng/ml.,灵敏度为0.14 ng/ml.。本试剂盒适用于基础科研中对大鼠模型脂代谢机制的研究,例如通过检测不同实验条件下(如基因干预、饮食调控或药物处理)血清或组织中的Apo - A1浓度变化,评估心血管疾病模型的病理进程或干预效果。为探索Apo - A1在脂质稳态及代谢性疾病中的功能提供高效研究工具。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    Apoa1Apolipoprotein A-I ELISA kit; Apo-AI ELISA kit; ApoA-I ELISA kit; Apolipoprotein A1) [Cleaved into: Proapolipoprotein A-I ELISA kit; ProapoA-I)] ELISA kit
  • 缩写:
    APOA1
  • Uniprot No.:
    P04639
  • 种属:
    Rattus norvegicus (Rat)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    0.14 ng/ml-100 ng/ml.
  • 灵敏度:
    0.14 ng/ml.
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Cardiovascular
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of rat Apo-A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1000

    Average %

    94


    Range %

    88-108


    1:2000

    Average %

    97


    Range %

    91-105


    1:4000

    Average %

    103


    Range %

    98-108


    1:8000

    Average %

    93


    Range %

    86-98


  • 回收率:

    The recovery of rat Apo-A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    95

    89-102


    EDTA plasma (n=4)

    89

    82-96


  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    100

    2.340

    2.434

    2.387

    2.275


    33.33

    1.892

    1.867

    1.880

    1.768


    11.11

    1.157

    1.128

    1.142

    1.031


    3.70

    0.625

    0.639

    0.632

    0.521


    1.23

    0.297

    0.311

    0.304

    0.192


    0.41

    0.188

    0.193

    0.190

    0.079


    0.14

    0.134

    0.140

    0.137

    0.025


    0

    0.108

    0.115

    0.112



  • 货期:
    3-5 working days

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