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Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. |
To assess the linearity of the assay, samples were spiked with high concentrations of rat Apo-A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1000 | Average % | 94 | ||||
Range % | 88-108 | |||||
1:2000 | Average % | 97 | ||||
Range % | 91-105 | |||||
1:4000 | Average % | 103 | ||||
Range % | 98-108 | |||||
1:8000 | Average % | 93 | ||||
Range % | 86-98 |
The recovery of rat Apo-A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 95 | 89-102 | ||||
EDTA plasma (n=4) | 89 | 82-96 |
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
ng/ml | OD1 | OD2 | Average | Corrected | ||
100 | 2.340 | 2.434 | 2.387 | 2.275 | ||
33.33 | 1.892 | 1.867 | 1.880 | 1.768 | ||
11.11 | 1.157 | 1.128 | 1.142 | 1.031 | ||
3.70 | 0.625 | 0.639 | 0.632 | 0.521 | ||
1.23 | 0.297 | 0.311 | 0.304 | 0.192 | ||
0.41 | 0.188 | 0.193 | 0.190 | 0.079 | ||
0.14 | 0.134 | 0.140 | 0.137 | 0.025 | ||
0 | 0.108 | 0.115 | 0.112 |
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