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Mouse Thrombospondin-2(THBS2) ELISA kit

  • 中文名: 小鼠血小板反应蛋白-2(THBS2)酶联免疫试剂盒
货号: IPD-EL023488MO
Price: ¥2500
数量:
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产品详情

  • 产品描述:
    小鼠血小板反应蛋白-2(THBS2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆.样本中的THBS2含量。THBS2是一个靶点。目前虽未给出其背景具体信息,但作为研究靶点,科学家可能围绕它探索其在生物体内的作用机制,如与疾病发生、发展的关联,在细胞信号传导、增殖分化等过程中扮演的角色,以寻找潜在治疗策略。试剂盒检测范围为6.25 ng/ml-400 ng/ml.,灵敏度为1.56 ng/ml.。科研人员可将其应用于心血管疾病模型、肿瘤微环境研究或组织修复机制探索等基础研究领域,通过检测不同病理状态下THBS2的表达动态,为相关分子机制解析提供数据支持。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    Thbs2 ELISA Kit; Tsp2 ELISA Kit; Thrombospondin-2 ELISA Kit
  • 缩写:
    THBS2
  • Uniprot No.:
    Q03350
  • 种属:
    Mus musculus (Mouse)
  • 样本类型:
    serum, plasma, tissue homogenates.
  • 检测范围:
    6.25 ng/ml-400 ng/ml.
  • 灵敏度:
    1.56 ng/ml.
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Cardiovascular
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse THBS2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    96


    Range %

    90-103


    1:2

    Average %

    102


    Range %

    97-106


    1:4

    Average %

    89


    Range %

    86-93


    1:8

    Average %

    94


    Range %

    89-98


  • 回收率:

    The recovery of mouse THBS2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    103

    98-107


    EDTA plasma (n=4)

    95

    90-111


  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    400

    2.366

    2.454

    2.410

    2.230


    200

    1.523

    1.633

    1.578

    1.398


    100

    0.974

    0.958

    0.966

    0.786


    50

    0.603

    0.632

    0.618

    0.438


    25

    0.421

    0.425

    0.423

    0.243


    12.5

    0.298

    0.286

    0.292

    0.112


    6.25

    0.233

    0.250

    0.242

    0.062


    0

    0.179

    0.180

    0.180




  • 货期:
    3-5 working days

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