首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠6酮前列腺素F1a(6-keto-PGF1a)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的6ketoPGF1a含量。试剂盒检测范围为25 pg/mL-1000 pg/mL，适用于心血管疾病模型、抗血栓药物研究、组织缺血再灌注损伤等科研场景的实验分析，为血管活性物质研究及药效评价提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：6ketoPGF1a
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：25 pg/mL-1000 pg/mL
灵敏度：25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse 6-K-PGF1a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 86-109 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 86-105 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-105 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 84-100 | |||||
回收率：
| The recovery of mouse 6-K-PGF1a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 86-107 | ||||
| EDTA plasma (n=4) | 94 | 83-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 10000.1300.1360.133 5000.2340.2200.227 2300.3590.3430.351 750.6180.5940.606 251.2041.2271.216 02.5922.6252.609 | ||||||
货期：3-5 working days
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