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产品描述：大鼠6-酮前列腺素F1α(6-keto-PGF1α)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、组织培养上清液样本中的6-keto-PGF1α含量。6-keto-PGF1α是一种由PGI2代谢生成的非活性产物，作为PGI2生物合成的有用标志物。其研究机制涉及HIF-1α、TXB2、6-Keto-PGF1α和HA的联合检测，以反映肝脏微循环障碍状况，其在慢性乙型肝炎等疾病诊断中具有重要意义。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，常用于研究血管舒缩调节、炎症反应及血栓形成等生理病理过程；支持心血管疾病模型、药物干预实验及炎症机制等基础研究，尤其适用于探索氧化应激、内皮损伤或抗血小板药物药效评价等领域；通过检测不同样本中6 - keto - PGF1α的动态变化，可为血管功能相关科研课题提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：6-keto-PGF1α,6-keto-PGF1 alpha
缩写：6-keto-PGF1α
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates, cell culture supernates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat 6-keto-PGF1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 80-90 | |||||
| 1:2 | Average % | 88 | ||||
| Range % | 82-95 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-95 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 84-97 | |||||
回收率：
| The recovery of rat 6-keto-PGF1α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 87-100 | ||||
| EDTA plasma (n=4) | 98 | 93-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.1512.1052.1282.03610001.7441.6591.7021.6105001.3281.2941.3111.2192500.9720.8960.9340.8421250.6270.6020.6150.52362.50.3130.2890.3010.20931.250.1960.1910.1940.10200.0950.0880.092 | ||||||
货期：3-5 working days
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