首页 / 产品 / ELISA试剂盒 / 其他ELISA试剂盒
产品描述：鱼促肾上腺皮质激素(ACTH)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ACTH含量。促肾上腺皮质激素（ACTH）由腺垂体分泌，能促进肾上腺皮质增生及肾上腺皮质激素合成与释放。其研究机制主要聚焦于调控糖皮质激素分泌、参与应激反应等。深入研究它能助力理解内分泌系统运行，为相关疾病治疗提供新思路。试剂盒检测范围为75 pg/mL-1200 pg/mL，对研究鱼类内分泌调节机制、环境应激适应性及养殖健康管理具有重要意义；支持科研人员开展鱼类生理状态评估、水环境毒理学研究及水产养殖中的激素水平监测；为探究鱼类神经内分泌系统功能、繁殖调控及应激病理机制提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：ACTH
种属：Fish
样本类型：serum, plasma, tissue homogenates
检测范围：75 pg/mL-1200 pg/mL
灵敏度：75 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of fish ACTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 105 | ||||
| Range % | 94-109 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 84-97 | |||||
| 1:4 | Average % | 101 | ||||
| Range % | 98-105 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 84-98 | |||||
回收率：
| The recovery of fish ACTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 82-97 | ||||
| EDTA plasma (n=4) | 94 | 90-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 12000.1490.1520.151 6000.3000.2880.294 3000.4770.4910.484 1500.8710.8890.880 751.8351.8871.861 | ||||||
货期：3-5 working days
×
