首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人ADAMTS样蛋白2(ADAMTSL2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ADAMTSL2含量。ADAMTSL2是一种位于9q34.2的基因，其编码的蛋白质属于ADAMTS（去整合素和金属蛋白酶与血小板反应蛋白基序）家族。该基因在多种生物中保守，其突变与地球物理发育不良有关。研究显示，ADAMTSL2与LTBP4的异常相互作用可能与青少年特发性脊柱侧凸有关，同时，ADAMTSL2蛋白和可溶性生物标记物在识别非酒精性脂肪性肝病（NAFLD）的高危非酒精性脂肪性肝炎和纤维化中具有重要作用。试剂盒检测范围为3.12 ng/mL-200 ng/mL，适用于基础科研中探索ADAMTSL2在发育调控、组织纤维化或代谢性疾病模型中的表达特征。为研究ADAMTSL2在疾病相关信号通路中的作用机制提供可靠工具，特别适用于生物标志物筛选、基因功能验证及病理机制研究等科研场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ADAMTS like 2 ELISA Kit; ADAMTS like protein 2 ELISA Kit; ADAMTS-like protein 2 ELISA Kit; ADAMTSL 2 ELISA Kit; ADAMTSL-2 ELISA Kit; ADAMTSL2 ELISA Kit; ATL2_HUMAN ELISA Kit; FLJ45164 ELISA Kit; KIAA0605 ELISA Kit
缩写：ADAMTSL2
Uniprot No.：Q86TH1
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 ng/mL-200 ng/mL
灵敏度：0.78 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ADAMTSL2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 99 | |||||
| Range % | 94-106 | ||||||
| 1:2 | Average % | 88 | |||||
| Range % | 84-93 | ||||||
| 1:4 | Average % | 92 | |||||
| Range % | 87-98 | ||||||
| 1:8 | Average % | 102 | |||||
| Range % | 98-106 | ||||||
回收率：
| The recovery of human ADAMTSL2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 91 | 87-96 | |||||
| EDTA plasma (n=4) | 86 | 81-92 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected2001.8541.9881.9211.8121001.2621.1921.2271.118500.7800.7330.7570.648250.4870.4510.4690.36012.50.3120.3340.3230.2146.250.2310.2170.2240.1153.120.1690.1600.1650.05600.1090.1080.109 | |||||||
货期：3-5 working days
×
