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产品描述：猪脂联素(ADP)酶联免疫试剂盒,为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ADP含量。ADP是一种重要的靶点。它是生物体内能量代谢的关键分子，参与细胞内多种生理过程。在研究机制上，ADP能与特定受体结合，触发细胞内信号传导通路，影响细胞功能。对ADP相关机制的研究，有助于深入了解疾病发生发展，为药物研发提供方向。试剂盒检测范围为1.875 μg/mL-30 μg/mL，特别适用于动物实验中对猪模型代谢机制、营养干预效果或肥胖相关病理的基础研究，可支持不同生物样本中ADP的动态监测，为畜牧营养学、比较医学等领域的分子机制探索提供有效工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：ADP
Uniprot No.：Q6PP07
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：1.875 μg/mL-30 μg/mL
灵敏度：1.875 μg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig ADP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 86-94 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 92-100 | |||||
| 1:4 | Average % | 83 | ||||
| Range % | 80-86 | |||||
| 1:8 | Average % | 91 | ||||
| Range % | 83-98 | |||||
回收率：
| The recovery of pig ADP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 87-99 | ||||
| EDTA plasma (n=4) | 93 | 91-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| μg/mlOD1OD2Average 300.1530.1650.159 150.2480.2400.244 7.50.5340.5110.523 3.751.1331.1351.134 1.8752.3402.2602.300 | ||||||
货期：3-5 working days
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