首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人胰淀粉酶(PAMY)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的AMY2A含量。AMY2A是一种编码α-淀粉酶的基因，主要在胰腺中表达。它通过水解寡糖和多糖中的1,4-α-葡萄糖苷键，催化淀粉和糖原的消化。研究显示，AMY2A与胰岛素抵抗和糖尿病有关，其表达水平与血糖水平相关。近期研究揭示了AMY2A在淀粉消化和血糖调节中的重要作用，为糖尿病治疗提供了新的靶点。试剂盒检测范围为47 mU/mL-3000 mU/mL，该产品适用于科研场景中胰腺外分泌功能评估、消化系统疾病机制研究或药物干预效果分析，例如在实验动物模型或体外细胞模型中追踪PAMY表达动态变化。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：AMY2A ELISA Kit; Pancreatic alpha-amylase ELISA Kit; PA ELISA Kit; EC 3.2.1.1 ELISA Kit; 1,4-alpha-D-glucan glucanohydrolase ELISA Kit
缩写：AMY2A
Uniprot No.：P04746
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：47 mU/mL-3000 mU/mL
灵敏度：11.75 mU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PAMY in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||
| Sample | Serum(n=4) | |||
| 1:1 | Average % | 95 | ||
| Range % | 89-99 | |||
| 1:2 | Average % | 102 | ||
| Range % | 96-107 | |||
| 1:4 | Average % | 94 | ||
| Range % | 87-98 | |||
| 1:8 | Average % | 86 | ||
| Range % | 81-92 |
回收率：
| The recovery of human PAMY spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 98 | 91-103 | |||||
| EDTA plasma (n=4) | 102 | 96-105 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| mU/mlOD1OD2AverageCorrected 30002.0662.1542.1101.992 15001.4961.5891.5431.425 7501.0071.0431.0250.907 3750.6340.6470.6410.523 187.50.3750.3820.3790.261 93.750.2670.2730.2700.152 46.880.1860.1890.1880.070 00.1170.1180.118 | |||||||
货期：3-5 working days
×
