关键信息
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基因名
SARS-CoV-2 NSP3
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简介
The multifunctional PL-PRO protein plays a critical role in viral RNA transcription and replication, providing the necessary proteases for multiprotein cleavage. It strategically blocks host translation by binding to the open head conformation of the 40S subunit, impeding ribosomal mRNA entry into the tunnel. NSP3 Protein, SARS-CoV-2 (I388-R745) is the recombinant virus-derived NSP3, expressed by E. coli, with tag-free.
- 应用
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别名
Replicase polyprotein 1a; ORF1a polyprotein; Severe acute respiratory syndrome coronavirus 2; 2019-nCoV; pp1a; Endonuclease; Hydrolase; Methyltransferase; Nuclease; Protease; RNA-binding; Thiol protease; Transferase
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种属
Virus
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表达系统
E. coli
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标签
Tag Free
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P0DTC1-1
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表达区间
I1206-R1563
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蛋白长度
Partial
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
SARS-CoV-2, the virus responsible for COVID-19, has garnered extensive research attention due to its significant impact on global health. Among its various proteins, the non-structural protein 3 (NSP3) is of particular interest due to its multifunctional roles in viral replication and host immune evasion. NSP3 is a large, modular protein that contains several functional domains, including a papain-like protease (PLpro) and a ubiquitin-like (Ubl) domain, which play critical roles in processing viral polyproteins and modulating host immune responses. Research into NSP3 has revealed its involvement in disrupting host antiviral signaling pathways, thus facilitating viral survival and replication. Additionally, NSP3 exhibits potential as a therapeutic target, as inhibiting its function could hinder viral propagation. The recombinant expression of NSP3 allows for detailed biochemical and structural studies, enabling researchers to elucidate its mechanisms of action and interactions with host proteins. Understanding NSP3 not only provides insights into the viral lifecycle but also assists in the development of antiviral strategies. Therefore, studying recombinant NSP3 is crucial for identifying potential drug targets and understanding the complex interplay between SARS-CoV-2 and the host immune system, ultimately contributing to the global effort in combating COVID-19.
