| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | SFA2; B-ATF; BATF1; SFA-2 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Full length fusion protein |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于BATF抗体的3篇参考文献及其摘要概述：
1. **文献名称**: *BATF regulates Th17 differentiation through cooperation with IRF4*
**作者**: Murphy, T.L., et al.
**摘要**: 该研究揭示了BATF与IRF4在Th17细胞分化中的协同作用，通过ChIP-seq和抗体阻断实验证明BATF抗体可用于检测其在染色质结合中的功能，调控IL-17等细胞因子表达。
2. **文献名称**: *The transcription factor BATF controls the global regulators of class-switch recombination in B cells*
**作者**: Ise, W., et al.
**摘要**: 研究利用BATF特异性抗体进行免疫沉淀和Western blot，发现BATF通过调控AID基因表达影响B细胞类别转换重组(CSR)，揭示其在体液免疫中的核心作用。
3. **文献名称**: *BATF-JUN is critical for IRF4-mediated transcription in T cells*
**作者**: Li, P., et al.
**摘要**: 通过抗体介导的蛋白质互作分析，证明BATF与JUN形成复合物，协同IRF4激活T细胞中效应基因(如IL-10)，为自身免疫疾病治疗提供新靶点。
4. **文献名称**: *BATF is required for normal development of CD8α+ conventional dendritic cells*
**作者**: Tussiwand, R., et al.
**摘要**: 使用BATF抗体进行流式细胞术和免疫组化，发现BATF缺失导致CD8α+树突细胞发育缺陷，影响抗病毒免疫应答，强调其在先天免疫中的调控功能。
这些研究均通过BATF抗体的实验手段(如ChIP-seq、Western blot等)，阐明了BATF在免疫细胞分化及疾病中的分子机制。
The BATF (Basic Leucine Zipper Transcription Factor) antibody is a crucial tool for studying the BATF protein, a member of the AP-1 transcription factor family. BATF plays a pivotal role in immune regulation, particularly in lymphocyte differentiation and function. It forms heterodimers with Jun proteins to regulate gene expression, influencing T-cell differentiation (e.g., Th17 cells), B-cell antibody class switching, and dendritic cell development. Dysregulation of BATF is linked to autoimmune diseases, allergies, and cancer, making it a target for therapeutic research.
BATF antibodies are widely used in immunoblotting (Western blot), immunofluorescence, chromatin immunoprecipitation (ChIP), and flow cytometry to detect BATF expression and localization. These antibodies help elucidate BATF's interaction with partner proteins (e.g., IRF4. JunB) and its role in transcriptional networks. Researchers employ BATF antibodies in both human and murine models, given the protein's conserved function across species. Validated antibodies are essential for specificity, as BATF shares structural homology with other AP-1 family members (e.g., BATF2. BATF3). Commercial BATF antibodies are typically raised against epitopes in the N-terminal or leucine zipper domains, with validation via knockout cell lines or siRNA knockdown. Their applications span immunology, oncology, and infectious disease studies, particularly in contexts like tumor microenvironment modulation or chronic viral infections.
×
