| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PAK4 |
| Uniprot No | O96013 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-426aa |
| 氨基酸序列 | MFGKRKKRVEISAPSNFEHRVHTGFDQHEQKFTGLPRQWQSLIEESARRPKPLVDPACITSIQPGAPKTIVRGSKGAKDGALTLLLDEFENMSVTRSNSLRRDSPPPPARARQENGMPEKPPGPRSPQREPQRVSHEQFRAALQLVVDPGDPRSYLDNFIKIGEGSTGIVCIATVRSSGKLVAVKKMDLRKQQRRELLFNEVVIMRDYQHENVVEMYNSYLVGDELWVVMEFLEGGALTDIVTHTRMNEEQIAAVCLAVLQALSVLHAQGVIHRDIKSDSILLTHDGRVKLSDFGFCAQVSKEVPRRKSLVGTPYWMAPELISRLPYGPEVDIWSLGIMVIEMVDGEPPYFNEPPLKAMKMIRDNLPPRLKNLHKVSPSLKGFLDRLLVRDPAQRATAAELLKHPFLAKAGPPASIVPLMRQNRTR |
| 预测分子量 | 63.9kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与PAK4重组蛋白相关的研究文献摘要概括：
1. **"Structural and Functional Characterization of PAK4 Recombinant Protein in Cancer Cell Signaling"**
- **作者**: Smith A, et al.
- **摘要**: 本研究通过大肠杆菌表达系统纯化PAK4重组蛋白，解析其激酶结构域的三维晶体结构，并验证其在Rho GTPase信号通路中对癌细胞迁移的调控作用。
2. **"PAK4 Recombinant Protein Enhances β-Catenin Stability via Phosphorylation in Wnt Signaling"**
- **作者**: Chen L, et al.
- **摘要**: 利用昆虫细胞表达的PAK4重组蛋白，揭示其通过磷酸化β-catenin增强Wnt信号通路活性，促进结直肠癌细胞增殖的分子机制。
3. **"Development of a High-Yield PAK4 Recombinant Protein Production System for Drug Screening"**
- **作者**: Wang Y, et al.
- **摘要**: 优化哺乳动物细胞中PAK4重组蛋白的表达和纯化工艺，建立基于该蛋白的激酶活性检测平台，用于筛选靶向PAK4的小分子抑制剂。
4. **"PAK4 Recombinant Protein Regulates Cytoskeletal Dynamics in Neuronal Development"**
- **作者**: Johnson R, et al.
- **摘要**: 通过体外实验证明重组PAK4蛋白通过磷酸化下游靶标蛋白调控神经元突触的形成和细胞骨架重组，影响神经发育过程。
(注：以上为示例性内容，实际文献需通过学术数据库检索确认。)
**Background of PAK4 Recombinant Protein**
PAK4 (p21-activated kinase 4) is a serine/threonine kinase belonging to the Group II PAK family, which plays pivotal roles in regulating cytoskeletal dynamics, cell survival, proliferation, and migration. It is activated by binding to small GTPases like Cdc42 and Rac1. which are critical mediators of signaling pathways involved in cancer progression, immune responses, and developmental processes. PAK4 is distinct from other PAK family members due to its unique structural features, including an N-terminal regulatory domain and a C-terminal kinase domain, enabling its specific interaction with downstream effectors.
Dysregulation of PAK4 is strongly linked to tumorigenesis, metastasis, and chemoresistance in various cancers, such as breast, pancreatic, and colorectal cancers. Overexpression of PAK4 correlates with poor prognosis, driving oncogenic signaling through pathways like MAPK, Wnt/β-catenin, and PI3K/AKT. Additionally, PAK4 is implicated in non-cancer pathologies, including neurological disorders and cardiovascular diseases, highlighting its broad therapeutic potential.
Recombinant PAK4 proteins are engineered to study its biochemical functions, substrate interactions, and inhibitor screening. Produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), these proteins retain kinase activity and structural integrity, enabling *in vitro* assays, structural studies (e.g., crystallography), and drug discovery. Tagged versions (e.g., His-, GST-, or FLAG-tagged) facilitate purification and detection. Researchers utilize PAK4 recombinant proteins to explore mechanisms of kinase activation, develop targeted inhibitors, and validate therapeutic candidates.
Overall, PAK4 recombinant proteins serve as essential tools for deciphering its role in cellular signaling and advancing precision medicine strategies against PAK4-driven diseases.
×
