| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | ELA3; ELA3A |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human CELA3A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CELA3A抗体的3篇代表性文献及其摘要概括：
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1. **文献名称**：*"Characterization of a monoclonal antibody specific for human pancreatic elastase 3A (CELA3A) and its application in immunohistochemistry"*
**作者**：Smith J, et al.
**摘要**：该研究开发了一种针对人CELA3A蛋白的单克隆抗体，验证了其在胰腺组织中的特异性。通过免疫组化实验证实，该抗体能有效标记胰腺腺泡细胞中的CELA3A表达，为胰腺外分泌功能的病理研究提供了工具。
2. **文献名称**：*"CELA3A as a potential biomarker in chronic pancreatitis: Validation by ELISA using a novel polyclonal antibody"*
**作者**：Lee S, et al.
**摘要**：研究团队通过制备兔源多克隆抗体，建立了检测CELA3A的ELISA方法。分析慢性胰腺炎患者血清发现CELA3A水平显著升高，提示其可能作为疾病进展的分子标志物。
3. **文献名称**：*"Role of CELA3A in pancreatic cancer progression and antibody-based detection in liquid biopsies"*
**作者**：Garcia M, et al.
**摘要**：探讨CELA3A在胰腺癌中的表达上调现象，并利用特异性抗体开发了基于外泌体的检测技术。研究发现，CELA3A高表达与肿瘤侵袭性相关，其抗体可用于液体活检中早期诊断的探索。
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注：上述文献信息为示例，实际研究中建议通过PubMed或Google Scholar以关键词“CELA3A antibody”检索最新文献，并优先选择近五年内发表的论文。
**Background of CELA3A Antibody**
CELA3A (Chymotrypsin-Like Elastase Family Member 3A) is a proteolytic enzyme primarily synthesized in the pancreas as an inactive zymogen (pro-CELA3A). It belongs to the elastase family of serine proteases, which play critical roles in digesting dietary proteins by cleaving peptide bonds. CELA3A is activated in the small intestine via trypsin-mediated cleavage, contributing to nutrient breakdown and enzyme activation cascades.
CELA3A antibodies are immunological tools designed to detect and quantify CELA3A protein expression in research or diagnostic settings. These antibodies are pivotal in studying pancreatic physiology, digestive disorders (e.g., pancreatitis), and pathologies linked to dysregulated protease activity, such as certain cancers or inflammatory conditions. Monoclonal and polyclonal CELA3A antibodies are commercially available, often validated for techniques like Western blotting, immunohistochemistry, and ELISA.
Recent studies highlight CELA3A's potential as a biomarker in pancreatic diseases, including diabetes and pancreatic ductal adenocarcinoma. However, cross-reactivity with homologous elastases (e.g., CELA3B) requires careful validation to ensure specificity. Advances in recombinant protein technology have improved antibody production, enhancing their reliability in both basic research and clinical applications.
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