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产品描述：猪脑钠素/脑钠尿肽(BNP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的BNP含量。BNP即脑钠肽，由心室肌细胞分泌。当心室容量负荷或压力负荷增加时，其分泌增加。它具有利钠、利尿、扩血管等作用，可拮抗肾素-血管紧张素-醛固酮系统。目前围绕BNP开展多项研究，旨在利用其特性改善心脏功能及应对相关疾病。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于心血管疾病基础研究、动物疾病模型评估、药效学实验中生物标志物动态监测等科研场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：NPPBNatriuretic peptides B ELISA kit; Gamma-brain natriuretic peptide) [Cleaved into: Brain natriuretic peptide 32 ELISA kit; BNP-32); Brain natriuretic peptide 26 ELISA kit; BNP-26)] ELISA kit
缩写：BNP
Uniprot No.：P07634
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig BNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||
| Sample | Serum(n=4) | ||
| 1:1 | Average % | 90 | |
| Range % | 86-95 | ||
| 1:2 | Average % | 94 | |
| Range % | 89-98 | ||
| 1:4 | Average % | 85 | |
| Range % | 80-93 | ||
| 1:8 | Average % | 98 | |
| Range % | 92-102 |
回收率：
| The recovery of pig BNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 91-102 | ||||
| EDTA plasma (n=4) | 88 | 84-92 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 20002.5512.5012.5262.392 10002.0031.9611.9821.848 5001.2711.2121.2421.108 2500.7420.7190.7310.597 1250.4070.4350.4210.287 62.50.3260.3030.3150.181 31.250.2270.2140.2210.087 00.1390.1280.134 | ||||||
货期：3-5 working days
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