| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | L20mt; MRP-L20 |
| WB Predicted band size | 17 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human MRPL20 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于MRPL20抗体的3篇参考文献示例(文献为模拟生成，仅供参考)：
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1. **标题**: *MRPL20 is essential for mitochondrial ribosome assembly and respiratory chain function*
**作者**: Antonicka, H., et al.
**摘要**: 本研究利用CRISPR敲除和MRPL20特异性抗体，揭示了MRPL20在线粒体核糖体大亚基组装中的关键作用。通过免疫沉淀和蛋白质组学分析，发现MRPL20缺失导致线粒体翻译缺陷及氧化磷酸化功能受损。
2. **标题**: *Dysregulation of MRPL20 expression in hepatocellular carcinoma correlates with poor prognosis*
**作者**: He, J., et al.
**摘要**: 通过免疫组化(使用兔源MRPL20抗体)分析肝癌组织样本，发现MRPL20表达显著下调，且低表达与患者生存率降低相关。进一步实验表明，MRPL20敲低通过干扰线粒体代谢促进癌细胞增殖。
3. **标题**: *A novel antibody-based assay for quantifying mitochondrial ribosomal proteins in human fibroblasts*
**作者**: Smith, K.L., & Patel, R.
**摘要**: 本文开发了一种基于MRPL20抗体的高灵敏度ELISA检测方法，用于定量人成纤维细胞中线粒体核糖体蛋白水平，为线粒体疾病的分子诊断提供了新工具。
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注：以上文献为示例，实际研究中请通过PubMed、Google Scholar等平台检索真实发表论文。
The MRPL20 antibody is a tool used to detect and study the mitochondrial ribosomal protein L20 (MRPL20), a component of the mitochondrial ribosome's large subunit. Mitochondrial ribosomes are distinct from cytoplasmic ribosomes and are essential for translating mitochondrial DNA (mtDNA)-encoded genes, which primarily produce subunits of the electron transport chain (ETC) complexes critical for oxidative phosphorylation (OXPHOS). MRPL20. encoded by nuclear DNA, is part of the protein machinery imported into mitochondria to assemble functional ribosomes. Dysregulation of mitochondrial ribosome components, including MRPL20. has been linked to mitochondrial dysfunction, which underlies various diseases such as neurodegenerative disorders, metabolic syndromes, and cancer.
The MRPL20 antibody is widely used in research to investigate mitochondrial protein synthesis, ribosome biogenesis, and cellular energy metabolism. It enables techniques like Western blotting, immunofluorescence, and immunohistochemistry to assess MRPL20 expression levels, subcellular localization, and interactions. Studies using this antibody have explored its role in cellular responses to stress, aging, and diseases like breast cancer, where altered mitochondrial translation may influence tumor progression or therapy resistance. By targeting MRPL20. researchers aim to uncover mechanisms connecting mitochondrial translation defects to pathological states, offering insights for therapeutic strategies targeting mitochondrial dysfunction.
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