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产品描述：人神经肽样蛋白C4orf48(C4orf48)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的C4orf48含量。C4orf48是一种位于人类4号染色体上的基因，编码一种神经肽样蛋白。该蛋白在神经系统的发育中表达，可能与智力障碍和神经退行性疾病有关。近期研究发现，C4orf48通过RNA结合机制促进肾脏纤维化，成为慢性肾脏病治疗的新靶点。试剂盒检测范围为0.625 ng/mL-40 ng/mL，适用于基础研究领域探索C4orf48在神经系统发育、神经退行性疾病模型中的表达调控，或结合组织样本分析其区域性分布特征。科研人员可通过检测体液或组织裂解液中的蛋白含量，研究其在特定生理病理过程中的潜在作用机制，为神经生物学研究提供实验工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：C4orf48 ELISA Kit; Chr4_55 ELISA Kit; Neuropeptide-like protein C4orf48 ELISA Kit
缩写：C4orf48
Uniprot No.：Q5BLP8
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human C4orf48 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 88-97 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 87-98 | |||||
| 1:8 | Average % | 103 | ||||
| Range % | 97-108 | |||||
回收率：
| The recovery of human C4orf48 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 86 | 81-92 | ||||
| EDTA plasma (n=4) | 102 | 97-107 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected402.5642.6892.6272.494202.1852.0772.1311.998101.4591.5241.4921.35950.8120.8280.8200.6872.50.4230.4320.4280.2951.250.2830.2940.2890.1560.6250.1860.1920.1890.05600.1320.1340.133 | ||||||
货期：3-5 working days
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