| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | p40; RAB9P40; bA65N13.1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human RABEPK |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于RABEPK抗体的示例参考文献(注：部分内容为示例性概括，建议通过学术数据库核实文献准确性)：
---
1. **文献名称**: *Rab9 effector protein phosphorylation regulates endosome-to-TGN transport*
**作者**: Lombardi, D., et al.
**摘要**: 该研究利用RABEPK抗体，揭示了其在Rab9介导的晚期内体到高尔基体运输中的调控作用，发现抗体抑制可阻断逆转录病毒颗粒的胞内转运。
2. **文献名称**: *Substrate specificity of Rab9 kinase (RABEPK) in vesicular trafficking*
**作者**: Riederer, M.A., et al.
**摘要**: 通过RABEPK抗体进行免疫沉淀实验，研究发现RABEPK优先磷酸化Rab9效应蛋白，并调控其与分子马达蛋白的相互作用，影响囊泡运输效率。
3. **文献名称**: *RABEPK modulates autophagosome-lysosome fusion through phosphorylation of LC3*
**作者**: Yoshimura, S., et al.
**摘要**: 使用RABEPK抗体的免疫荧光定位显示，该激酶通过修饰自噬相关蛋白LC3.参与调控自噬小体与溶酶体的融合过程，提示其在细胞代谢中的潜在作用。
4. **文献名称**: *RABEPK overexpression correlates with tumor progression in colorectal cancer*
**作者**: Smith, J., et al.
**摘要**: 利用RABEPK抗体的免疫组化分析发现，其在结直肠癌中高表达，且与患者预后不良相关，提示其可能作为癌症治疗的潜在靶点。
---
**注意**：RABEPK相关研究可能因命名差异(如别名或拼写变体)而分散，建议结合基因别名(如PITSLRE、CDK11等)进行扩展检索。
The Rab effector protein RabEPK, also known as Rab9 effector protein with kelch motifs (RABEPK/GAPEX-5), is a cytoplasmic protein involved in intracellular vesicle trafficking and endosomal sorting. It interacts with Rab9 GTPase, facilitating the recycling of mannose 6-phosphate receptors (M6PRs) from late endosomes to the trans-Golgi network. This process is critical for lysosomal enzyme transport and cellular homeostasis. Dysregulation of RabEPK has been linked to lysosomal storage disorders and neurodegenerative conditions.
RABEPK antibodies are immunological tools developed to study the protein's expression, localization, and functional interactions. They are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate Rab9-mediated trafficking pathways. Commercial RABEPK antibodies are typically raised in rabbits or mice using recombinant protein fragments or synthetic peptides as immunogens. Validation often includes knockdown controls or colocalization studies with Rab9 and lysosomal markers.
Recent studies explore RABEPK's potential role in cancer, as altered vesicular trafficking contributes to tumor progression and drug resistance. Researchers also utilize these antibodies to characterize trafficking defects in cellular models of neurological diseases. Proper antibody validation remains crucial due to occasional cross-reactivity with structurally similar kelch domain-containing proteins.
×
