| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | PDHA; PDHAD; PHE1A; PDHCE1A |
| WB Predicted band size | 43 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human PDHA1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与PDHA1抗体相关的参考文献示例(内容为虚构，仅作格式参考)：
1. **文献名称**：*PDHA1 expression in human cancers: implications for metabolic reprogramming*
**作者**：Smith J, et al.
**摘要**：本研究通过免疫组化(IHC)和Western blot分析PDHA1抗体在多种肿瘤中的表达，发现PDHA1低表达与糖酵解增强和患者预后不良相关，提示其作为代谢标志物的潜力。
2. **文献名称**：*Antibody-specific detection of phosphorylated PDHA1 in mitochondrial metabolism regulation*
**作者**：Chen L, et al.
**摘要**：开发了一种特异性识别PDHA1磷酸化位点(Ser293)的抗体，验证其在细胞模型中的敏感性，并揭示PDHA1磷酸化状态对线粒体丙酮酸代谢的关键调控作用。
3. **文献名称**：*PDHA1 deficiency screening using a novel monoclonal antibody*
**作者**：Wang Y, et al.
**摘要**：报道一种新型PDHA1单克隆抗体的制备与验证，通过ELISA和免疫荧光技术成功应用于丙酮酸脱氢酶缺乏症患者的基因突变与蛋白表达关联性研究。
(注：以上文献为模拟示例，实际引用需查询PubMed等数据库获取真实文献。)
The PDHA1 antibody is a crucial tool in studying the pyruvate dehydrogenase complex (PDC), a central enzyme system in cellular energy metabolism. PDHA1 encodes the E1 alpha subunit of PDC, located on the X chromosome (Xp22.12). This mitochondrial matrix enzyme catalyzes the irreversible decarboxylation of pyruvate to acetyl-CoA, linking glycolysis to the tritricarboxylic acid (TCA) cycle. PDHA1 antibodies specifically detect this 43 kDa protein, enabling researchers to investigate its expression patterns, post-translational modifications (particularly phosphorylation at Ser293 regulating activity), and subcellular localization.
These antibodies have become essential in studying metabolic disorders like pyruvate dehydrogenase deficiency (PDHD), where PDHA1 mutations account for 80% of cases. They're also used in cancer research, as many tumors show altered PDC activity affecting the Warburg effect. Neuroscience applications include investigating PDHA1's role in maintaining neurotransmitter balance and neuronal survival. Common techniques utilizing PDHA1 antibodies include Western blotting, immunohistochemistry, and immunofluorescence. Recent studies explore its potential as a biomarker in metabolic syndrome and neurodegenerative diseases. Validation remains critical due to phosphorylation state variations and homologous subunits in the PDH family.
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