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产品描述：猪清道夫受体富含半胱氨酸1蛋白M130(CD163)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CD163含量。CD163是一种细胞表面糖蛋白，在单核细胞和巨噬细胞上表达。其可被特定分子识别激活，在炎症反应、组织修复等生理过程中发挥重要作用。目前研究聚焦于其作为炎症标志物及治疗靶点，探索调节其功能来干预相关疾病。试剂盒检测范围为93.75 ng/mL-6000 ng/mL，该产品适用于动物模型研究、免疫病理机制探索以及感染性疾病进程中巨噬细胞活化状态的评估，为科研人员在猪源样本中分析CD163的表达动态、验证免疫调控通路及评估炎症反应程度提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CD163 ELISA Kit; M130Scavenger receptor cysteine-rich type 1 protein M130 ELISA Kit; CD antigen CD163) [Cleaved into: Soluble CD163 ELISA Kit; sCD163)] ELISA Kit
缩写：CD163
Uniprot No.：Q2VL90
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：93.75 ng/mL-6000 ng/mL
灵敏度：23.4 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig CD163 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 86 | |||||
| Range % | 82-90 | ||||||
| 1:2 | Average % | 94 | |||||
| Range % | 91-97 | ||||||
| 1:4 | Average % | 103 | |||||
| Range % | 100-106 | ||||||
| 1:8 | Average % | 99 | |||||
| Range % | 95-103 | ||||||
回收率：
| The recovery of pig CD163 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 92-98 | |||||
| EDTA plasma (n=4) | 89 | 86-92 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected60002.1112.2282.1702.00330001.3651.4551.4101.24315000.8450.8680.8570.6907500.5550.5740.5650.3983750.3690.3490.3590.192187.50.2820.2630.2730.10693.750.2350.2300.2330.06600.1700.1630.167 | |||||||
货期：3-5 working days
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