首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠内皮唾酸蛋白(CD248)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CD248含量。CD248，又称内皮唾液酸蛋白，是一种在肿瘤相关成纤维细胞和周细胞中表达的肿瘤标志物。其研究机制涉及调节Wnt信号通路，促进血管生成和肿瘤生长，在多种肿瘤中发挥重要作用。试剂盒检测范围为7.81 pg/mL-500 pg/mL，该产品适用于科研领域对血管生成机制、肿瘤微环境调控或纤维化疾病模型的探索性研究，为体外定量分析CD248在生理或病理状态下的动态变化提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Cd248 ELISA Kit; Tem1 ELISA Kit; Endosialin ELISA Kit; Tumor endothelial marker 1 ELISA Kit; CD antigen CD248 ELISA Kit
缩写：CD248
Uniprot No.：Q91V98
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：7.81 pg/mL-500 pg/mL
灵敏度：1.95 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse CD248 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:100 | Average % | 95 | |||||
| Range % | 90-99 | ||||||
| 1:200 | Average % | 102 | |||||
| Range % | 96-107 | ||||||
| 1:400 | Average % | 93 | |||||
| Range % | 89-98 | ||||||
| 1:800 | Average % | 86 | |||||
| Range % | 80-92 | ||||||
回收率：
| The recovery of mouse CD248 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 97 | 93-102 | |||||
| EDTA plasma (n=4) | 98 | 94-103 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected5002.6432.7812.7122.6252501.7651.8221.7941.7071251.1211.2111.1661.07962.50.7210.7100.7160.62931.250.4330.4480.4410.35415.620.2760.2820.2790.1927.810.1820.1880.1850.09800.0860.0880.087 | |||||||
货期：3-5 working days
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