Mouse CD9 antigen(CD9) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-EL004969MO

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
小鼠CD9抗原(CD9)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CD9含量。CD9 作为一个重要靶点，在细胞表面广泛表达。其背景上与细胞黏附、迁移等活动密切相关。研究机制方面，它可参与多种信号通路的调控，调节细胞间相互作用。对 CD9 的深入研究有助于探索疾病发生发展机制并开发相关治疗策略。试剂盒检测范围为4.69 pg/mL-300 pg/mL，本产品适用于科研领域探究CD9在细胞间相互作用、肿瘤微环境调控、神经再生等方向的功能机制，为体外模型、动物实验或原代细胞研究中CD9蛋白表达动态提供可靠检测工具，满足基础研究中对生物标志物的定量分析需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Cd9; CD9 antigen; CD antigen CD9
缩写：
CD9
Uniprot No.：
P40240
种属：
Mus musculus (Mouse)
样本类型：
serum, plasma, tissue homogenates, cell lysates
检测范围：
4.69 pg/mL-300 pg/mL
灵敏度：
1.17 pg/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Immunology
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse CD9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	99
	Range %	92-105
1:2	Average %	108
	Range %	103-114
1:4	Average %	97
	Range %	92-103
1:8	Average %	93
	Range %	86-98

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
300	3.208	3.134	3.171	3.028
150	2.727	2.767	2.747	2.604
75	1.940	1.988	1.964	1.821
37.5	1.052	1.029	1.040	0.897
18.75	0.537	0.521	0.529	0.386
9.375	0.286	0.293	0.289	0.146
4.69	0.209	0.199	0.204	0.061
0	0.145	0.141	0.143