首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠钙黏蛋白5(CDH5)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CDH5含量。CDH5即钙黏蛋白 5，是一种重要的细胞黏附分子。其在血管内皮细胞中高度表达，参与维持内皮细胞连接和血管完整性。研究机制方面，它与肿瘤血管生成、内皮屏障功能等密切相关，是肿瘤治疗、血管相关疾病研究的潜在靶点。试剂盒检测范围为0.235 ng/mL-15 ng/mL，适用于内皮细胞功能研究、血管相关疾病模型评估或药物干预下CDH5表达水平变化的定量分析；可为血管生物学、病理机制及药物开发等研究提供精准的CDH5定量检测方案；在血管生成、炎症反应及肿瘤转移等研究中具有重要价值本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Cdh5; Cadherin-5; Vascular endothelial cadherin; VE-cadherin; CD antigen CD144
缩写：CDH5
Uniprot No.：P55284
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：0.235 ng/mL-15 ng/mL
灵敏度：0.058 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse CDH5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 92 | ||||
| Range % | 88-96 | |||||
| 1:200 | Average % | 95 | ||||
| Range % | 91-98 | |||||
| 1:400 | Average % | 100 | ||||
| Range % | 96-105 | |||||
| 1:800 | Average % | 94 | ||||
| Range % | 87-98 | |||||
回收率：
| The recovery of mouse CDH5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 99 | 92-108 | ||||
| EDTA plasma (n=4) | 94 | 90-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 152.5132.4132.4632.359 7.51.6981.5981.6481.544 3.750.9700.9400.9550.851 1.880.4990.4890.4940.390 0.940.3260.3160.3210.217 0.470.2170.2070.2120.108 0.2350.1470.1450.1460.042 00.1050.1030.104 | ||||||
货期：3-5 working days
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