| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/5000 | Human,Mouse,Rat |
| Aliases | CDC18L; HsCDC6; MGORS5; HsCDC18 |
| WB Predicted band size | 63 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human CDC6 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CDC6抗体的3篇参考文献的简要列举，涵盖其分子机制及临床应用：
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1. **文献名称**：*CDC6 as a biomarker for prognosis and DNA replication in colorectal cancer*
**作者**：Xiao-Xin Li 等
**摘要**：该研究通过免疫组化(使用CDC6抗体)分析了结直肠癌组织中CDC6的表达水平，发现CDC6的高表达与肿瘤分期、转移及患者预后不良显著相关。结果提示CDC6可能作为结直肠癌的潜在预后标志物。
2. **文献名称**：*CDC6 regulates cisplatin resistance in gastric cancer via modulating DNA repair and apoptosis*
**作者**：Hua Zhang 等
**摘要**：研究利用CDC6抗体探究其在胃癌细胞中的功能，发现CDC6过表达通过增强DNA损伤修复能力导致顺铂耐药，而敲低CDC6可增加化疗敏感性，为靶向CDC6的癌症治疗提供依据。
3. **文献名称**：*The role of CDC6 in licensing human chromatin for DNA replication*
**作者**：J. Julian Blow 等
**摘要**：该基础研究通过CDC6抗体结合染色质免疫沉淀(ChIP)技术，阐明了CDC6在DNA复制起始中的关键作用，揭示其与ORC复合物协同调控复制起始点的分子机制。
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以上文献分别从临床预后、治疗耐药性及基础分子机制角度探讨了CDC6的功能，均涉及CDC6抗体的实验应用。如需具体文献来源(期刊、年份)，建议通过PubMed或Google Scholar以标题及作者名进一步检索。
The CDC6 antibody is a crucial tool for studying the cell division cycle 6 (CDC6) protein, a key regulator of DNA replication and cell cycle progression. CDC6 is essential for the assembly of the pre-replication complex (pre-RC) during the G1 phase, ensuring precise initiation of DNA replication. Structurally, it contains an AAA+ ATPase domain and collaborates with the origin recognition complex (ORC) and Cdt1 to load mini-chromosome maintenance (MCM) helicases onto replication origins. Dysregulation of CDC6 is linked to genomic instability, uncontrolled proliferation, and tumorigenesis, with overexpression observed in cancers like breast, lung, and colorectal.
CDC6 antibodies are widely used in techniques such as Western blotting, immunofluorescence, and immunohistochemistry to detect protein expression, localization, and dynamics across cell cycle stages. They help investigate CDC6's roles in replication licensing, checkpoint control, and its interplay with tumor suppressors (e.g., p53) or oncogenic pathways. Monoclonal antibodies (e.g., clone DCS-180) offer high specificity, while polyclonal versions may detect conserved epitopes across species. Validation often includes knockout/knockdown controls to confirm target specificity.
Research using CDC6 antibodies has highlighted its potential as a diagnostic marker for cancer prognosis and therapeutic targeting, particularly in disrupting replication in rapidly dividing cells. These studies underscore its importance in both basic cell biology and translational oncology.
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