首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠C型凝集素结构域家族18成员A(CLEC18A)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CLEC18A含量。CLEC18A是一种C型凝集素，广泛分布于人体各个器官，尤其在肝脏和骨髓细胞中含量最高。其研究机制主要涉及其在抗病毒免疫反应中的作用，与TLR3结合增强免疫信号传递，并通过基因变异影响免疫反应。此外，CLEC18A在肝脏纤维化和脂肪肝的病理过程中也扮演重要角色。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于探索CLEC18A在感染性疾病、自身免疫模型或炎症机制研究中的动态表达规律，亦可应用于基因编辑动物模型中蛋白表达水平的验证，为免疫调控机制及分子信号通路研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Clec18a ELISA kit; Mrcl ELISA kit; MrlpC-type lectin domain family 18 member A ELISA kit; Mannose receptor-like protein ELISA kit
缩写：CLEC18A
Uniprot No.：Q7TSQ1
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse CLEC18A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 84 | |||||
| Range % | 80-89 | ||||||
| 1:2 | Average % | 95 | |||||
| Range % | 91-99 | ||||||
| 1:4 | Average % | 86 | |||||
| Range % | 83-89 | ||||||
| 1:8 | Average % | 98 | |||||
| Range % | 94-103 | ||||||
回收率：
| The recovery of mouse CLEC18A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 89 | 85-94 | |||||
| EDTA plasma (n=4) | 102 | 98-106 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected 202.5462.5002.5232.402 101.9472.0101.9791.858 51.4191.3681.3941.273 2.50.8310.8420.8370.716 1.250.5250.5360.5310.410 0.6250.3570.3710.3640.243 0.3120.2670.2510.2590.138 00.1220.1200.121 | |||||||
货期：3-5 working days
×
