| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PPIL4 |
| Uniprot No | Q8WUA2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-512aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMAVLLETTLGDVVIDLYTEERPRACLNFLK LCKIKYYNYCLIHNVQRDFIIQTGDPTGTGRGGESIFGQLYGDQASFFEA EKVPRIKHKKKGTVSMVNNGSDQHGSQFLITTGENLDYLDGVHTVFGEVT EGMDIIKKINETFVDKDFVPYQDIRINHTVILDDPFDDPPDLLIPDRSPE PTREQLDSGRIGADEEIDDFKGRSAEEVEEIKAEKEAKTQAILLEMVGDL PDADIKPPENVLFVCKLNPVTTDEDLEIIFSRFGPIRSCEVIRDWKTGES LCYAFIEFEKEEDCEKAFFKMDNVLIDDRRIHVDFSQSVAKVKWKGKGGK YTKSDFKEYEKEQDKPPNLVLKDKVKPKQDTKYDLILDEQAEDSKSSHSH TSKKHKKKTHHCSEEKEDEDYMPIKNTNQDIYREMGFGHYEEEESCWEKQ KSEKRDRTQNRSRSRSRERDGHYSNSHKSKYQTDLYERERSKKRDRSRSP KKSKDKEKSKYR |
| 预测分子量 | 59 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PPIL4重组蛋白的3篇参考文献示例(注：以下内容为模拟文献，实际文献需通过学术数据库检索确认)：
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1. **文献名称**：*PPIL4 regulates glioblastoma migration via MAPK/ERK pathway*
**作者**：Zhang Y, et al.
**摘要**：本研究揭示了重组人PPIL4蛋白通过调控MAPK/ERK信号通路促进胶质母细胞瘤细胞的侵袭和迁移，并通过体外实验验证了PPIL4与ERK1/2的相互作用。
2. **文献名称**：*Structural and functional analysis of recombinant PPIL4 in protein folding*
**作者**：Liu H, et al.
**摘要**：通过X射线晶体学解析了重组PPIL4蛋白的三维结构，发现其异构酶活性依赖于保守的催化位点，并在体外验证了其对含脯氨酸基序底物的折叠促进作用。
3. **文献名称**：*PPIL4 interacts with USP9X to stabilize oncogenic proteins in cancer*
**作者**：Wang L, et al.
**摘要**：研究发现重组PPIL4蛋白与去泛素化酶USP9X形成复合物，通过抑制靶蛋白(如MCL-1)的泛素化降解，促进肿瘤细胞存活，为癌症治疗提供了新靶点。
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如需真实文献，建议通过PubMed、Google Scholar等平台以关键词“PPIL4 recombinant protein”或“PPIL4 structure/function”检索近年发表的文章。
**Background of PPIL4 Recombinant Protein**
PPIL4 (Peptidylprolyl Isomerase Like 4) is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family, which plays critical roles in protein folding, conformational regulation, and cellular processes such as signal transduction and apoptosis. PPIL4 is characterized by its conserved PPIase domain, enabling it to catalyze the isomerization of peptide bonds preceding proline residues, a rate-limiting step in protein structural maturation. While its paralogs (e.g., PPIA/CYPA) are well-studied in immunoregulation and disease contexts, PPIL4 remains less characterized, though emerging studies suggest its involvement in mRNA splicing, cell cycle regulation, and cancer progression.
Recombinant PPIL4 protein is engineered for research applications, typically expressed in *E. coli* or mammalian systems to ensure proper folding and post-translational modifications. The purified protein retains enzymatic activity and is utilized in biochemical assays, structural studies (e.g., X-ray crystallography), and interaction analyses to map its binding partners. Its role in the spliceosome complex, particularly in modulating pre-mRNA processing, has sparked interest in its potential links to genetic disorders and malignancies.
PPIL4's overexpression in certain cancers, such as hepatocellular carcinoma, correlates with poor prognosis, suggesting its utility as a biomarker or therapeutic target. Additionally, studies in model organisms highlight its developmental importance, with knockout models exhibiting defects in neural and immune systems. Despite these advances, the mechanistic details of PPIL4's functions and regulatory networks remain under exploration. The availability of recombinant PPIL4 facilitates ongoing research to decode its biological significance and translational potential in health and disease.
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