首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠Ⅳ型胶原(Col Ⅳ)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的Col Ⅳ含量。Col Ⅳ，即IV型胶原，是一种主要的细胞外基质蛋白，在多种生理过程中扮演关键角色，如血管生成、组织修复和细胞粘附。其研究机制涉及与MMP-12等酶的相互作用，以及通过降解Col-Ⅳ来诱导血管生成等生物学过程。试剂盒检测范围为0.78 ng/mL-50 ng/mL，适用于科研领域中小鼠模型的相关研究，例如通过检测血清/血浆中Col Ⅳ浓度评估全身性基底膜代谢状态，或通过分析肝、肾等组织匀浆的Col Ⅳ表达水平，探究器官特异性纤维化进展及药物干预效果。可满足体外实验中对Col Ⅳ动态变化的定量分析需求，为基底膜相关机制研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Collagen Type 4,Col 4,_x000D_ Collagen Type IV,Col IV
缩写：Col Ⅳ
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：0.78 ng/mL-50 ng/mL
灵敏度：0.195 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse COL Ⅳ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 100 | ||||
| Range % | 97-103 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 84-93 | |||||
| 1:4 | Average % | 88 | ||||
| Range % | 84-92 | |||||
| 1:8 | Average % | 101 | ||||
| Range % | 97-106 | |||||
回收率：
| The recovery of mouse COL Ⅳ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 85 | 81-90 | ||||
| EDTA plasma (n=4) | 96 | 93-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 502.4572.5692.5132.334 251.6121.6081.6101.431 12.50.9490.9680.9590.780 6.250.5370.5480.5430.364 3.120.3940.3750.3850.206 1.560.3040.3120.3080.129 0.780.2590.2620.2610.082 00.1780.1790.179 | ||||||
货期：3-5 working days
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