首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠I型胶原α1链(COL1A1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的COL1A1含量。COL1A1是一种编码I型胶原蛋白α1链的基因，存在于骨骼、肌腱和皮肤等结缔组织中，提供强度和支持。其突变与多种疾病相关，如成骨不全症、埃勒斯-当洛斯综合征等。研究COL1A1有助于理解胶原蛋白合成和维持组织结构的分子机制。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于体外实验中对小鼠模型在纤维化疾病、骨骼发育异常或皮肤再生等研究场景下的 COL1A1 动态变化分析；支持科研人员高效评估药物干预、基因编辑或病理模型中胶原代谢的调控效应，为探索组织微环境重塑机制提供可靠工具；满足基础研究与转化医学中对胶原蛋白代谢通路的高通量筛选需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Col1a1 ELISA kit; Cola1Collagen alpha-1(I) chain ELISA kit; Alpha-1 type I collagen ELISA kit
缩写：COL1A1
Uniprot No.：P11087
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse COL1A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 81-92 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 95-105 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 85-95 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 91-101 | |||||
回收率：
| The recovery of mouse COL1A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 88 | 82-92 | ||||
| EDTA plasma (n=4) | 100 | 90-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 202.5582.5992.5792.386 102.0082.1112.0601.867 51.3891.3421.3661.173 2.50.8430.8280.8360.643 1.250.5030.5400.5220.329 0.6250.3770.3840.3810.188 0.3120.2780.2680.2730.080 00.1950.1910.193 | ||||||
货期：3-5 working days
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