Recombinant Human Recombinant Human PPP1R2 protein

PPP1R2 (Protein Phosphatase 1 Regulatory Subunit 2) is a regulatory protein that interacts with protein phosphatase 1 (PP1), a serine/threonine phosphatase critical for cellular processes such as cell cycle progression, signal transduction, and metabolism. As a member of the PP1 regulatory subunit family, PPP1R2 modulates PP1 activity by binding to its catalytic subunit, influencing substrate specificity and localization. PP1 itself is involved in dephosphorylation events that counterbalance kinase-driven phosphorylation, ensuring precise control over pathways like glycogen synthesis, muscle contraction, and apoptosis.

Recombinant PPP1R2 is produced using biotechnological methods, often through expression in bacterial (e.g., *E. coli*) or eukaryotic systems (e.g., mammalian cells), followed by purification via affinity chromatography. This engineered protein retains the functional domains necessary for PP1 interaction, enabling researchers to study its inhibitory mechanisms and regulatory roles *in vitro*. Structurally, PPP1R2 contains conserved motifs for PP1 binding, and its recombinant form is pivotal for dissecting molecular interactions through techniques like co-immunoprecipitation or enzymatic assays.

Research on PPP1R2 has implications for understanding diseases linked to PP1 dysregulation, including cancer, neurodegenerative disorders, and metabolic syndromes. Its overexpression or mutation may disrupt phosphorylation equilibria, contributing to pathological signaling. Additionally, recombinant PPP1R2 serves as a tool for screening therapeutic agents targeting PP1-related pathways. Despite progress, questions remain about its tissue-specific functions and post-translational modifications, underscoring the need for further structural and functional studies to unlock its diagnostic or therapeutic potential.