首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人和肽素(copeptin)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、尿液、组织培养上清液、组织匀浆样本中的copeptin含量。Copeptin是一种由精氨酸加压素原羧基末端产生的肽，作为加压素（抗利尿激素，ADH）的替代物，其水平在多种疾病中升高，如心血管疾病、肾病和肺部疾病等。Copeptin的检测简便、快速，可有效地反映内源性AVP的水平，近年来，其在疾病诊断和预后评估方面的作用成为研究热点。试剂盒检测范围为78 pg/mL-5000 pg/mL，适用于心血管疾病机制研究、脓毒症病理模型探索、水盐代谢相关基础实验等领域，支持体外实验中copeptin水平的动态监测及生物标志物相关性分析，为应激相关疾病的基础研究提供高效检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：copeptin
种属：Homo sapiens (Human)
样本类型：serum, urine, cell culture supernates, tissue homogenates
检测范围：78 pg/mL-5000 pg/mL
灵敏度：19.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human Copeptin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 82-95 | |||||
| 1:2 | Average % | 99 | ||||
| Range % | 92-105 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 90-106 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 91-102 | |||||
回收率：
| The recovery of human Copeptin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 88-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected50002.5352.7522.6442.53325002.0122.1792.0961.98512501.5981.5991.5991.4886250.8470.8670.8570.7463120.4780.5020.4900.3791560.3360.3560.3460.235780.2370.2210.2290.11800.1090.1120.111 | ||||||
本试剂盒所含材料：
A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-Human copeptin antibody.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled copeptin antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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