| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PREP |
| Uniprot No | P48147 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-710aa |
| 氨基酸序列 | LSLQYPDVYRDETAVQDYHGHKICDPYAWLEDPDSEQTKAFVEAQNKITVPFLEQCPIRGLYKERMTELYDYPKYSCHFKKGKRYFYFYNTGLQNQRVLYVQDSLEGEARVFLDPNILSDDGTVALRGYAFSEDGEYFAYGLSASGSDWVTIKFMKVDGAKELPDVLERVKFSCMAWTHDGKGMFYNSYPQQDGKSDGTETSTNLHQKLYYHVLGTDQSEDILCAEFPDEPKWMGGAELSDDGRYVLLSIREGCDPVNRLWYCDLQQESSGIAGILKWVKLIDNFEGEYDYVTNEGTVFTFKTNRQSPNYRVINIDFRDPEESKWKVLVPEHEKDVLEWIACVRSNFLVLCYLHDVKNILQLHDLTTGALLKTFPLDVGSIVGYSGQKKDTEIFYQFTSFLSPGIIYHCDLTKEELEPRVFREVTVKGIDASDYQTVQIFYPSKDGTKIPMFIVHKKGIKLDGSHPAFLYGYGGFNISITPNYSVSRLIFVRHMGGILAVANIRGGGEYGETWHKGGILANKQNCFDDFQCAAEYLIKEGYTSPKRLTINGGSNGGLLVAACANQRPDLFGCVIAQVGVMDMLKFHKYTIGHAWTTDYGCSDSKQHFEWLVKYSPLHNVKLPEADDIQYPSMLLLTADHDDRVVPLHSLKFIATLQYIVGRSRKQSNPLLIHVDTKAGHGAGKPTAKVIEEVSDMFAFIARCLNVDWIP |
| 预测分子量 | 88.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PREP(脯氨酸寡肽酶)重组蛋白的3篇参考文献，按研究内容分类整理：
1. **文献名称**：Prolyl endopeptidase from Sphingomonas capsulata: Heterologous expression and structural analysis
**作者**：H. Yamaguchi et al.
**摘要**：研究报道了在E. coli系统中高效表达Sphingomonas来源的PREP重组蛋白，并通过X射线晶体学解析了其三维结构，揭示了底物特异性机制。
2. **文献名称**：Recombinant human prolyl endopeptidase: Production and therapeutic potential in neurodegenerative diseases
**作者**：M. García-Horsman et al.
**摘要**：开发了CHO细胞表达的人源PREP重组蛋白纯化工艺，并在阿尔茨海默病模型中验证了其通过降解异常蛋白聚集物的治疗潜力。
3. **文献名称**：Engineering thermostable PREP variants for industrial applications
**作者**：K. Takahashi & S. Watanabe
**摘要**：通过定向进化技术改造PREP重组蛋白的热稳定性，获得在60℃仍保持活性的突变体，显著提升了该酶在食品加工中的工业适用性。
注：以上文献为虚拟示例，实际研究中建议通过PubMed(https://pubmed.ncbi.nlm.nih.gov)搜索关键词"prolyl endopeptidase recombinant"获取最新文献，近三年相关研究多集中在蛋白工程改造(如2022年Nature Catalysis刊载的理性设计研究)及神经退行性疾病治疗应用领域。
**Background of Recombinant PREP Protein**
Prolyl endopeptidase (PREP), also known as prolyl oligopeptidase, is a serine protease that cleaves peptide bonds at the C-terminal side of proline residues in short peptides. Initially identified in the 1970s, PREP has garnered attention for its role in neuropeptide metabolism, including the processing of hormones like oxytocin, vasopressin, and neurotensin. It is implicated in neurological disorders such as Alzheimer’s disease, Parkinson’s disease, and schizophrenia, where dysregulation of peptide signaling may contribute to pathogenesis. Structurally, PREP consists of a catalytic domain with a characteristic α/β-hydrolase fold and a seven-bladed β-propeller domain that regulates substrate access.
Recombinant PREP proteins are engineered using heterologous expression systems (e.g., *E. coli*, yeast, or mammalian cells*) to enable large-scale production for functional and structural studies. Advances in recombinant DNA technology have allowed precise control over protein variants, facilitating investigations into substrate specificity, enzymatic mechanisms, and inhibitor design. PREP inhibitors are explored as potential therapeutics, given their ability to modulate neuropeptide levels and influence cognitive or behavioral outcomes.
Research on recombinant PREP also extends to cancer and inflammation, as PREP activity correlates with cellular proliferation and immune response pathways. The protein’s dual role in peptide processing and protein-protein interactions underscores its biological versatility. However, challenges remain in understanding its physiological versus pathological functions, necessitating high-purity recombinant PREP for crystallography, drug screening, and cellular assays. Overall, recombinant PREP serves as a critical tool for unraveling its multifaceted roles and therapeutic potential.
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