| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | HSD-PLA2 |
| WB Predicted band size | 44 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human PAFAH2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于PAFAH2抗体的参考文献示例(内容为虚构，仅供格式参考)：
1. **文献名称**: *"PAFAH2 regulates lipid metabolism and inflammatory responses in macrophages"*
**作者**: Zhang Y, et al.
**摘要**: 研究利用PAFAH2特异性抗体，通过免疫印迹和免疫荧光技术，揭示了PAFAH2在巨噬细胞中通过水解血小板活化因子(PAF)调控脂质代谢并抑制炎症反应的机制。
2. **文献名称**: *"Structural insights into PAFAH2 enzyme complex by cryo-EM"*
**作者**: Tanaka K, et al.
**摘要**: 通过PAFAH2抗体纯化酶复合物，结合冷冻电镜技术解析其三维结构，阐明了PAFAH2催化活性位点的构象变化及其底物特异性。
3. **文献名称**: *"PAFAH2 deficiency exacerbates neuronal injury in Alzheimer's disease models"*
**作者**: Chen L, et al.
**摘要**: 研究使用PAFAH2抗体检测转基因小鼠脑组织中的蛋白表达，发现PAFAH2缺失通过增加氧化应激加重淀粉样斑块沉积和神经元损伤。
(注：上述文献为模拟内容，实际引用请查阅PubMed或SciFinder等学术数据库。)
The PAFAH2 (Platelet-Activating Factor Acetylhydrolase 2) antibody is a tool used to detect and study the PAFAH2 enzyme, a member of the phospholipase A2 superfamily. PAFAH2. also known as PLA2G7B, is a cytoplasmic enzyme that hydrolyzes platelet-activating factor (PAF), a potent lipid mediator involved in inflammation, immune responses, and vascular homeostasis. Unlike its isoform PAFAH1. which forms a heterotrimeric complex, PAFAH2 functions as a monomer and exhibits distinct substrate preferences, including oxidized phospholipids associated with oxidative stress. Its role in mitigating PAF-mediated inflammatory processes and modulating lipid metabolism has linked it to conditions like atherosclerosis, neurodegenerative diseases, and cancer.
PAFAH2 antibodies are typically developed using immunogenic peptides or recombinant protein fragments, and they enable researchers to investigate the enzyme’s expression, localization, and function in various tissues and disease models. These antibodies are validated for applications such as Western blotting, immunohistochemistry, and immunofluorescence, often with species specificity for human, mouse, or rat samples. Commercially available PAFAH2 antibodies are crucial for elucidating its regulatory mechanisms, interactions with signaling pathways, and potential therapeutic targeting. Proper validation via knockout controls or siRNA knockdown ensures specificity, as cross-reactivity with related enzymes (e.g., PAFAH1) must be ruled out.
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