首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠胱抑素F(CST7)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CST7含量。小鼠胱抑素F（CST7）是一种具有半胱氨酸型内肽酶抑制活性的蛋白质，参与肽酶活性的负调控，在抑制半胱氨酸型内肽酶活性、负调控小胶质细胞激活和正调控髓鞘化等过程中发挥作用，其表达与神经炎症和神经病理性疼痛有关。试剂盒检测范围为0.156 ng/mL-10 ng/mL，该产品适用于神经科学、免疫学等领域的基础研究，可应用于实验动物模型（如神经损伤、炎症性疾病模型）中胱抑素F的动态监测，为探究其与疾病发生发展的关联提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Cst7Cystatin-F ELISA Kit; Cystatin-7 ELISA Kit; Cystatin-like metastasis-associated protein ELISA Kit; CMAP ELISA Kit; Leukocystatin ELISA Kit
缩写：CST7
Uniprot No.：O89098
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse CST7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 89 | |||||
| Range % | 84-93 | ||||||
| 1:2 | Average % | 100 | |||||
| Range % | 95-105 | ||||||
| 1:4 | Average % | 87 | |||||
| Range % | 83-90 | ||||||
| 1:8 | Average % | 104 | |||||
| Range % | 100-107 | ||||||
回收率：
| The recovery of mouse CST7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 86 | 83-89 | |||||
| EDTA plasma (n=4) | 95 | 91-99 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected102.5472.6012.5742.45252.1202.0322.0761.9542.51.3941.4021.3981.2761.250.8200.8050.8130.6910.6250.5170.5310.5240.4020.3120.3250.3260.3260.2040.1560.2390.2420.2410.11900.1210.1230.122 | |||||||
货期：3-5 working days
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