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Human monocyte interferon gamma inducing factor,MIG ELISA Kit

  • 中文名： 人γ干扰素诱导单核细胞因子(MIG/CXCL9)ELISA Kit
货号: IPD-E09024h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人γ干扰素诱导单核细胞因子(MIG/CXCL9)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆.样本中的CXCL9含量。CXCL9是一个有研究价值的靶点。它属于趋化因子家族，主要由免疫细胞等分泌。在机体免疫防御、炎症反应等过程中发挥作用。研究多聚焦于其在肿瘤微环境中对免疫细胞的趋化机制，以及与肿瘤生长、转移和免疫逃逸的关系。试剂盒检测范围为39 pg/ml-2500 pg/ml.，灵敏度为9.75 pg/ml.。适用于体外研究领域，如感染性疾病模型、免疫调控机制探索、药物干预效果评估等科研场景，为炎症反应研究、免疫细胞趋化功能分析及生物标志物筛查提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    C-X-C motif chemokine 9 ELISA Kit; chemokine (C-X-C motif) ligand 9 ELISA Kit; CMK ELISA Kit; crg-10 ELISA Kit; CXCL9 ELISA Kit; CXCL9_HUMAN ELISA Kit; gamma interferon induced monokine ELISA Kit; Gamma-interferon-induced monokine ELISA Kit; HuMIG ELISA Kit; MIG ELISA Kit; monokine induced by gamma interferon ELISA Kit; monokine induced by interferon gamma ELISA Kit; Monokine induced by interferon-gamma ELISA Kit; SCYB9 ELISA Kit; Small inducible cytokine B9 ELISA Kit; small inducible cytokine subfamily B member 9 ELISA Kit; Small-inducible cytokine B9 ELISA Kit
  • 缩写：
    CXCL9
  • Uniprot No.：
    Q07325
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates.
  • 检测范围：
    39 pg/ml-2500 pg/ml.
  • 灵敏度：
    9.75 pg/ml.
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human MIG/CXCL9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    95


    Range %

    91-99


    1:2

    Average %

    87


    Range %

    83-90


    1:4

    Average %

    104


    Range %

    100-108


    1:8

    Average %

    88


    Range %

    85-92


  • 回收率：

    The recovery of human MIG/CXCL9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    102

    97-106


    EDTA plasma (n=4)

    110

    107-113


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected


    2500

    2.151

    2.204

    2.178

    2.070


    1250

    1.647

    1.602

    1.625

    1.517


    625

    1.045

    1.104

    1.075

    0.967


    312

    0.701

    0.731

    0.716

    0.608


    156

    0.411

    0.425

    0.418

    0.310


    78

    0.253

    0.256

    0.255

    0.147


    39

    0.150

    0.157

    0.154

    0.046


    0

    0.107

    0.109

    0.108




  • 货期：
    3-5 working days

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