| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/10-1/50 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | LAM; TSC4; PPP1R160 |
| WB Predicted band size | 201 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human TSC2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于TSC2抗体的3篇参考文献示例(文献信息为模拟，仅供参考)：
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1. **文献名称**：*TSC2 Antibody Validation in mTOR Signaling Studies*
**作者**：Smith J, et al.
**摘要**：本研究通过CRISPR敲除TSC2基因的细胞模型，利用特异性TSC2抗体进行Western blot和免疫荧光分析，验证了抗体在检测TSC2蛋白表达及定位中的可靠性，并揭示了TSC2缺失导致mTORC1信号通路的异常激活。
2. **文献名称**：*TSC2 Expression in Renal Cell Carcinoma: Immunohistochemical Analysis*
**作者**：Lee H, et al.
**摘要**：通过免疫组化方法，使用TSC2抗体对肾细胞癌组织样本进行检测，发现TSC2蛋白表达水平与肿瘤恶性程度呈负相关，提示TSC2在抑制肿瘤发生中的潜在作用。
3. **文献名称**：*Role of TSC2 in Autophagy Regulation: Antibody-Based Detection*
**作者**：Garcia R, et al.
**摘要**：研究采用TSC2抗体结合免疫印迹技术，证明TSC2通过调控mTOR通路影响自噬活性，并在神经元细胞模型中观察到TSC2缺失导致自噬相关蛋白LC3-II的异常积累。
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**注**：以上文献信息为示例，实际引用时需核对真实文献来源(如PubMed、Google Scholar等数据库)。建议使用关键词“TSC2 antibody validation”、“TSC2 immunohistochemistry”或“TSC2 mTOR signaling”检索最新研究。
**Background of TSC2 Antibody**
The TSC2 antibody targets the tuberous sclerosis complex 2 (TSC2) protein, encoded by the *TSC2* gene. TSC2. also known as tuberin, is a tumor suppressor that forms a heterodimeric complex with TSC1 (hamartin). This complex regulates the mTOR (mechanistic target of rapamycin) signaling pathway by acting as a GTPase-activating protein (GAP) for Rheb (Ras homolog enriched in brain), thereby inhibiting mTOR complex 1 (mTORC1) activity. Dysregulation of TSC2 due to mutations or loss of function leads to constitutive mTOR activation, contributing to tuberous sclerosis complex (TSC), a genetic disorder characterized by benign tumors in multiple organs, neurological deficits, and epilepsy.
TSC2 antibodies are widely used in research to study protein expression, localization, and post-translational modifications (e.g., phosphorylation at Ser939 or Thr1462) in cell and tissue samples. They are critical tools for investigating mTOR pathway dynamics, cellular growth, autophagy, and metabolic regulation. Commercially available TSC2 antibodies include polyclonal and monoclonal variants, often validated for applications like Western blotting, immunohistochemistry, and immunofluorescence.
In disease research, TSC2 antibodies help assess TSC2 expression loss in TSC-associated lesions or cancers and evaluate therapeutic responses to mTOR inhibitors. Challenges include ensuring antibody specificity due to homology between TSC2 and other proteins, necessitating validation via knockout cell lines or siRNA. Ongoing studies continue to refine TSC2 antibody utility in translational research and diagnostics.
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