首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人A型肝配蛋白受体(EPHA2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的EPHA2含量。EPHA2，即A型肝配蛋白受体-2，是一种受体酪氨酸激酶，参与细胞迁移、粘附、增殖和分化。其研究机制涉及与多种肝配蛋白A配体的双向信号传导，以及与gHgL蛋白的结合，在γ疱疹病毒侵入机制中发挥重要作用。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于体外研究EPHA2在肿瘤生物学、细胞信号转导等领域的表达变化，或用于药物研发中靶点作用机制及干预效果的评估。支持科研人员在分子生物学、病理机制探索等基础研究中高效获取定量数据。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ARCC2 ELISA Kit; AW545284 ELISA Kit; CTPA ELISA Kit; CTPP1 ELISA Kit; CTRCT6 ELISA Kit; EC 2.7.10.1 ELISA Kit; Eck ELISA Kit; Eph receptor A2 ELISA Kit; EPHA2 ELISA Kit; EPHA2_HUMAN ELISA Kit; Ephrin receptor ELISA Kit; Ephrin receptor EphA2 ELISA Kit; Ephrin type A receptor 2 ELISA Kit; Ephrin type-A receptor 2 ELISA Kit; Epithelial cell kinase ELISA Kit; Epithelial cell receptor protein tyrosine kinase ELISA Kit; Myk 2 ELISA Kit; Myk2 ELISA Kit; Sek 2 ELISA Kit; Sek2 ELISA Kit; Soluble EPHA2 variant 1 ELISA Kit; Tyrosine protein kinase receptor ECK ELISA Kit; Tyrosine-protein kinase receptor ECK ELISA Kit; Tyrosine-protein kinase receptor MPK-5 ELISA Kit; Tyrosine-protein kinase receptor SEK-2 ELISA Kit
缩写：EPHA2
Uniprot No.：P29317
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human EPHA2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 85-97 | |||||
| 1:2 | Average % | 100 | ||||
| Range % | 95-105 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 89-99 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 90-100 | |||||
回收率：
| The recovery of human EPHA2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 89-101 | ||||
| EDTA plasma (n=4) | 92 | 85-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.4642.5752.5202.40151.7681.8891.8291.7102.51.2361.2651.2511.1321.250.7120.7660.7390.6200.6250.4070.4350.4210.3020.3120.2540.2640.2590.1400.1560.2280.2450.2370.11800.1160.1210.119 | ||||||
货期：3-5 working days
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