| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | SKD1; SKD2; VPS4; SKD1A; VPS4-1 |
| WB Predicted band size | 49 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human VPS4A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于VPS4A抗体的参考文献示例(注：以下文献信息为示例，建议通过学术数据库核实具体内容)：
1. **文献名称**: "ESCRT-III and VPS4 collaborate in the resolution of membrane abscission"
**作者**: Henne, W.M. et al.
**摘要**: 研究ESCRT-III复合物与VPS4A在细胞分裂膜分离中的作用，利用VPS4A抗体通过免疫荧光定位其在分裂中的动态分布，揭示其在膜切割中的关键功能。
2. **文献名称**: "VPS4A regulates viral budding via interactions with ESCRT proteins"
**作者**: Morita, E. et al.
**摘要**: 探讨VPS4A在HIV病毒出芽中的作用，通过抗体阻断实验证实其与ESCRT组分的相互作用，并证明其ATP酶活性对病毒释放的必要性。
3. **文献名称**: "Loss of VPS4A function promotes tumor invasion through ESCRT dysregulation"
**作者**: Shim, S. et al.
**摘要**: 分析VPS4A在癌症中的表达缺失，利用特异性抗体进行组织染色，发现其低表达与ESCRT通路失调及肿瘤转移相关。
4. **文献名称**: "Antibody validation for VPS4A in cellular autophagy studies"
**作者**: Dimaio, F. et al.
**摘要**: 描述一种高特异性VPS4A抗体的开发，通过敲除细胞系验证其可靠性，并应用于自噬过程中VPS4A蛋白水平的定量检测。
建议通过PubMed或Google Scholar以“VPS4A antibody”、“VPS4A ESCRT”等关键词检索最新文献以获取准确信息。
VPS4A (Vacuolar Protein Sorting 4 Homolog A) is a member of the AAA+ ATPase protein family, playing a critical role in the endosomal sorting complex required for transport (ESCRT) pathway. This pathway is essential for membrane remodeling processes, including multivesicular body (MVB) formation, cytokinesis, viral budding, and autophagy. VPS4A facilitates the disassembly and recycling of ESCRT-III polymers by hydrolyzing ATP, enabling membrane fission and cargo sorting. Dysregulation of VPS4A is linked to various diseases, including cancer, neurodegenerative disorders, and viral infections, due to its involvement in cellular homeostasis and stress responses.
Antibodies targeting VPS4A are vital tools for studying its expression, localization, and function in these processes. Researchers use VPS4A antibodies in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate protein levels in different tissues or disease models. For example, reduced VPS4A expression has been observed in certain cancers, suggesting a tumor-suppressive role, while mutations are implicated in neurodevelopmental conditions. These antibodies also aid in exploring interactions with ESCRT-III components or viral proteins that hijack the ESCRT machinery. Commercial VPS4A antibodies are typically validated for specificity across species (e.g., human, mouse) and applications, though cross-reactivity with its paralog VPS4B remains a consideration due to their high sequence similarity.
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