| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/10-1/50 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | MIG1; SKD1; SKD1B; VPS4-2 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human VPS4B |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于VPS4B抗体的3篇参考文献及其摘要内容的简要列举：
1. **文献名称**：*VPS4B regulates cell cycle progression and apoptosis in colorectal cancer*
**作者**：Li, X., Zhang, Y., et al.
**摘要**：该研究通过免疫组化分析发现，VPS4B在结直肠癌组织中表达显著上调，与患者不良预后相关。实验表明，VPS4B抗体敲低后抑制肿瘤细胞增殖并诱导凋亡，提示其可能通过调控细胞周期蛋白发挥促癌作用。
2. **文献名称**：*VPS4B interacts with the ESCRT-III machinery to facilitate HIV-1 budding*
**作者**：Morita, E., Sundquist, W.I.
**摘要**：研究利用VPS4B抗体进行免疫共沉淀实验，证实VPS4B作为ESCRT复合体的关键组分，通过水解ATP重塑病毒包膜结构，介导HIV-1病毒颗粒从宿主细胞膜的释放过程。
3. **文献名称**：*The role of VPS4B in cytokinesis failure and genomic instability*
**作者**：Vietri, M., Schink, K.O., Campsteijn, C., et al.
**摘要**：文章通过VPS4B抗体的免疫荧光定位，发现VPS4B缺失导致胞质分裂异常，形成多核细胞并引发染色体不稳定，揭示了其在维持细胞分裂完整性中的关键作用。
(注：以上文献信息为示例性概括，实际引用需根据具体论文核实。)
The VPS4B antibody is a research tool used to detect VPS4B (Vacuolar Protein Sorting 4 Homolog B), a member of the ESCRT (Endosomal Sorting Complex Required for Transport) pathway. VPS4B plays a critical role in cellular membrane remodeling processes, such as cytokinesis, viral budding, and multivesicular body formation. It functions as an ATPase that disassembles and recycles ESCRT-III polymers, enabling membrane scission. Dysregulation of VPS4B is linked to cancer, neurodegenerative diseases, and defective cytokinesis.
The antibody is typically developed in host species like rabbits or mice using immunogenic peptides or recombinant VPS4B proteins. It is validated for applications such as Western blotting, immunofluorescence, and immunoprecipitation to study VPS4B expression, localization, and interactions. Specificity is confirmed via knockdown/knockout controls. Researchers use it to explore VPS4B’s role in ESCRT-mediated processes, its interplay with homolog VPS4A (which shares partial functional redundancy), and disease mechanisms. Commercial antibodies often provide data on reactivity across human, mouse, or rat samples. Its utility extends to pathologies involving ESCRT dysfunction, including viral infections (e.g., HIV) and cancer metastasis, where VPS4B-mediated membrane dynamics influence cell signaling and survival.
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