首页 / 产品 / ELISA试剂盒 / 其他ELISA试剂盒
产品描述：犬红细胞生成素(EPO)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的EPO含量。EPO即促红细胞生成素，是一种糖蛋白激素，由肾脏分泌。其背景在于它能促进红细胞生成，维持机体正常的氧运输能力。研究机制方面，EPO作用于骨髓中红系祖细胞表面受体，促进红系祖细胞增殖、分化和成熟，增加红细胞数量，在治疗贫血等方面有重要意义。试剂盒检测范围为1.56 mIU/mL-100 mIU/mL，适用于科研场景中犬类贫血机制研究、肾脏功能与造血系统关联性分析、药物干预效果评价或疾病模型（如肾衰竭、肿瘤性贫血）的EPO动态监测本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：EPOErythropoietin ELISA kit
缩写：EPO
Uniprot No.：P33707
种属：Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 mIU/mL-100 mIU/mL
灵敏度：0.39 mIU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of dog EPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 97 | |||||
| Range % | 91-105 | ||||||
| 1:2 | Average % | 89 | |||||
| Range % | 80-97 | ||||||
| 1:4 | Average % | 93 | |||||
| Range % | 86-100 | ||||||
| 1:8 | Average % | 95 | |||||
| Range % | 88-100 | ||||||
回收率：
| The recovery of dog EPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 97 | 83-102 | |||||
| EDTA plasma (n=4) | 95 | 85-102 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| mIU/mlOD1OD2AverageCorrected1001.9021.8931.8981.725501.5051.5181.5121.339251.0871.1461.1170.94412.50.8040.7890.7970.6246.250.4990.5120.5060.3333.120.3420.3510.3470.1741.560.2550.2610.2580.08500.1700.1760.173 | |||||||
货期：3-5 working days
×
