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产品描述：猪红细胞生成素(EPO)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的EPO含量。EPO（促红细胞生成素）是一种糖蛋白激素，主要在肾脏和肝脏产生，控制红细胞的生成。其研究机制涉及调节红细胞前体细胞的分化，并参与神经保护和伤口愈合等过程。EPO在治疗贫血和某些疾病中发挥重要作用，但其副作用和兴奋剂使用也备受关注。试剂盒检测范围为1 mIU/mL-40 mIU/mL，适用于动物生理机制研究、疾病模型构建、药物干预效果评估等科研领域，尤其为猪的血液系统疾病研究、肾脏功能探索及缺氧适应性分析提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：EPOErythropoietin ELISA Kit
缩写：EPO
Uniprot No.：P49157
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：1 mIU/mL-40 mIU/mL
灵敏度：0.5 mIU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig EPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 83-91 | |||||
| 1:2 | Average % | 88 | ||||
| Range % | 84-93 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 95-101 | |||||
| 1:8 | Average % | 100 | ||||
| Range % | 97-103 | |||||
回收率：
| The recovery of pig EPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 95-102 | ||||
| EDTA plasma (n=4) | 93 | 90-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| mIU/ml.OD1OD2Average 402.6872.6992.693 201.9801.8791.930 101.1051.0971.101 50.6040.6340.619 20.2710.2930.282 10.1320.1340.133 | ||||||
货期：3-5 working days
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