首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠嗜酸性粒细胞过氧化物酶(EPO)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的EPX含量。小鼠嗜酸性粒细胞过氧化物酶（EPO）是一种调节骨代谢的关键酶，通过抑制破骨细胞和促进成骨细胞功能，维持骨稳态。其作用机制涉及降低破骨细胞活性氧水平，抑制RANK-MAPK信号通路，并可能影响成骨细胞分化和矿化。试剂盒检测范围为0.156 ng/mL-10 ng/mL，可助力探索嗜酸性粒细胞介导的免疫反应机制、评估实验性炎症模型中的细胞活化状态，或用于药物干预下EPO动态变化的监测，为免疫学、病理学及药理学研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Epx ELISA Kit; Eper ELISA Kit; Eosinophil peroxidase ELISA Kit; EPO ELISA Kit; EC 1.11.1.7) [Cleaved into: Eosinophil peroxidase light chain; Eosinophil peroxidase heavy chain] ELISA Kit
缩写：EPX
Uniprot No.：P49290
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse EPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 84-92 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:4 | Average % | 99 | ||||
| Range % | 95-104 | |||||
| 1:8 | Average % | 102 | ||||
| Range % | 98-107 | |||||
回收率：
| The recovery of mouse EPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 92-98 | ||||
| EDTA plasma (n=4) | 96 | 94-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.2692.2982.2842.08651.6741.6981.6861.4882.51.0811.0981.0900.8921.250.7290.7690.7490.5510.6250.4370.4570.4470.2490.3120.3880.3630.3760.1780.1560.2830.3030.2930.09500.1970.1980.198 | ||||||
货期：3-5 working days
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