首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人表皮生长因子受体2(sp185/Her2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的ERBB2含量。ERBB2又名HER2，是一种原癌基因，在细胞生长、增殖和存活中起重要作用。其过度表达或扩增可导致肿瘤发生发展。研究机制主要围绕其信号通路，通过抑制该靶点活性、阻断信号传导等，为癌症治疗提供新策略，已成为肿瘤治疗重要研究方向。试剂盒检测范围为0.156 ng/mL-10 ng/mL，该产品适用于体外科研实验中对HER2表达水平的动态监测，例如肿瘤微环境研究、药物干预效果评估或分子信号通路机制探索，其优化的反应体系兼容多种生物样本类型，可为细胞实验、动物模型及临床前研究提供可靠的定量数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog ELISA Kit; C erb B2/neu protein ELISA Kit; CD340 ELISA Kit; CD340 antigen ELISA Kit; Cerb B2/neu protein ELISA Kit; CerbB2 ELISA Kit; Erb b2 receptor tyrosine kinase 2 ELISA Kit; ErbB-2 proto-oncogene ELISA Kit; ERBB2 ELISA Kit; ERBB2_HUMAN ELISA Kit; HER 2 ELISA Kit; HER 2/NEU ELISA Kit; HER2 ELISA Kit; Herstatin ELISA Kit; Human epidermal growth factor receptor 2 ELISA Kit; Metastatic lymph node gene 19 protein ELISA Kit; MLN 19 ELISA Kit; MLN19 ELISA Kit; NEU ELISA Kit; NEU proto oncogene ELISA Kit; Neuro/glioblastoma derived oncogene homolog ELISA Kit; Neuroblastoma/glioblastoma derived oncogene homolog ELISA Kit; NGL ELISA Kit; p185erbB2 ELISA Kit; Proto-oncogene c-ErbB-2 ELISA Kit; Proto-oncogene Neu ELISA Kit; Receptor tyrosine-protein kinase erbB-2 ELISA Kit; TKR1 ELISA Kit; Tyrosine kinase type cell surface receptor HER2 ELISA Kit; Tyrosine kinase-type cell surface receptor HER2 ELISA Kit; V erb b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog) ELISA Kit; V erb b2 avian erythroblastic leukemia viral oncogene homolog 2 ELISA Kit; V erb b2 avian erythroblastic leukemia viral oncoprotein 2 ELISA Kit; V erb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) ELISA Kit; V erb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog ELISA Kit; Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) ELISA Kit
缩写：ERBB2
Uniprot No.：P04626
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.04 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human sp185/HER2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:5 | Average % | 103 | |||||
| Range % | 94-107 | ||||||
| 1:10 | Average % | 98 | |||||
| Range % | 91-101 | ||||||
| 1:20 | Average % | 92 | |||||
| Range % | 86-95 | ||||||
| 1:40 | Average % | 106 | |||||
| Range % | 95-110 | ||||||
回收率：
| The recovery of human sp185/HER2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 102 | 94-106 | |||||
| EDTA plasma (n=4) | 94 | 88-98 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected102.5112.4762.4942.38052.0071.9551.9811.8672.51.4971.4521.4751.3611.251.0401.0071.0240.9100.6250.6550.6420.6490.5350.3120.4160.4040.4100.2960.1560.2530.2480.2510.13700.1120.1160.114 | |||||||
货期：3-5 working days
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