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产品描述：犬内皮素1(ET-1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的EDN1含量。EDN1即内皮素-1，是一种具有强烈而持久血管收缩作用的活性肽。在生理上，它参与维持心血管稳态。研究机制方面，聚焦其与心血管、呼吸系统疾病等关联，探讨其信号通路及作用靶点，期望开发针对性药物干预治疗。试剂盒检测范围为3.12 pg/mL-200 pg/mL，适用于科研领域对犬血清、血浆及组织匀浆样本中 ET - 1 含量的精准分析；适用于实验室对犬模型在基础研究、药物作用机制探索或疾病模型构建中的 ET - 1 动态监测本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：EDN1Endothelin-1 ELISA kit; ET-1 ELISA kit; Preproendothelin-1 ELISA kit; PPET1) [Cleaved into: Big endothelin-1] ELISA kit
缩写：EDN1
Uniprot No.：P13206
种属：Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 pg/mL-200 pg/mL
灵敏度：0.78 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of dog ET-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 85-96 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 90-99 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 81-96 | |||||
| 1:8 | Average % | 100 | ||||
| Range % | 91-108 | |||||
回收率：
| The recovery of dog ET-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 87 | 82-96 | ||||
| EDTA plasma (n=4) | 97 | 82-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected2002.9542.9892.9722.8611002.3882.3982.3932.282501.7581.7891.7741.663251.0351.0691.0520.94112.50.5020.5430.5230.4126.250.2710.2680.2700.1593.120.1840.1690.1770.06600.1120.1100.111 | ||||||
货期：3-5 working days
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