rat Endothelin 1,ET-1 ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-E06979r

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：大鼠内皮素1(ET-1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、细胞裂解物、组织匀浆样本中的EDN1含量。EDN1，即内皮素-1，是一种内皮源性血管收缩肽，在血管调节中发挥重要作用。它作为G蛋白偶联受体EDNRA和EDNRB的可能配体，在肾小球系膜细胞中启动信号级联，调节血管张力。研究显示，EDN1在血管生理学中扮演关键角色，参与多种细胞过程。试剂盒检测范围为1.25 pg/mL-80 pg/mL，在科研领域，本试剂盒可应用于高血压动物模型研究、血管功能评估、药物干预效果验证以及内皮细胞相关机制探索，为心血管疾病、肾脏病理或呼吸系统疾病的基础研究提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Edn1 ELISA Kit; Endothelin-1 ELISA Kit; ET-1 ELISA Kit; Preproendothelin-1 ELISA Kit; PPET1) [Cleaved into: Big endothelin-1] ELISA Kit
缩写：EDN1
Uniprot No.：P22388
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell lysates, tissue homogenates
检测范围：1.25 pg/mL-80 pg/mL
灵敏度：0.31 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat ET-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	84-91
1:2	Average %	96
	Range %	91-102
1:4	Average %	84
	Range %	81-87
1:8	Average %	92
	Range %	88-96

回收率：

The recovery of rat ET-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-96
EDTA plasma (n=4)	95	90-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/mlOD1OD2AverageCorrected802.0852.0862.0861.951401.5401.6491.5951.460201.1111.1561.1340.999100.7180.7500.7340.59950.4070.4050.4060.2712.50.2310.2370.2340.0991.250.1810.1730.1770.04200.1290.1400.135