首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠埃兹蛋白(EZR)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的EZR含量。小鼠埃兹蛋白（EZR）是ERM家族成员，主要定位于细胞膜下，与肌动蛋白丝结合调节细胞骨架重组，影响细胞形态和运动。EZR还参与细胞信号传导，如RhoA、PI3K/AKT等，调节细胞增殖、凋亡和代谢。EZR异常表达与多种癌症、免疫缺陷和代谢性疾病相关。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，适用于基础科研中探究EZR在细胞极性调控、上皮 - 间质转化（EMT）等生物学过程中的作用机制，或用于疾病模型中EZR表达水平与病理表型的关联性分析，为细胞生物学、肿瘤学及免疫学领域的研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ezr ELISA kit; Vil2Ezrin ELISA kit; Cytovillin ELISA kit; Villin-2 ELISA kit; p81 ELISA kit
缩写：EZR
Uniprot No.：P26040
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse EZR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 86 | |||||
| Range % | 80-91 | ||||||
| 1:2 | Average % | 96 | |||||
| Range % | 91-102 | ||||||
| 1:4 | Average % | 103 | |||||
| Range % | 97-108 | ||||||
| 1:8 | Average % | 93 | |||||
| Range % | 89-97 | ||||||
回收率：
| The recovery of mouse EZR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 96 | 92-102 | |||||
| EDTA plasma (n=4) | 87 | 80-92 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected40002.4122.5262.4692.28420002.1132.1482.1311.94610001.6321.5621.5971.4125000.9780.9960.9870.8022500.5460.5310.5390.3541250.3690.3610.3650.18062.50.2680.2740.2710.08600.1860.1840.185 | |||||||
货期：3-5 working days
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