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产品描述：人凝血因子XIII A1 (F13A1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的F13A1含量。人凝血因子XIII A1（F13A1）是凝血级联反应中最后一个被激活的酶原，由两个A亚基和两个B亚基组成的异四聚体。A亚基具有催化功能，参与纤维蛋白交联和稳定，而B亚基作为载体保护A亚基。F13A1缺陷会导致出血倾向、伤口愈合缺陷和习惯性流产。试剂盒检测范围为0.625 ng/mL-40 ng/mL，适用于基础医学研究中分析F13A1在凝血机制中的作用、评估疾病模型（如血栓形成或出血性疾病）中该因子的表达变化，或用于药物开发中筛选调控凝血功能的化合物，为探索凝血相关分子机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：bA525O21.1 (coagulation factor XIII; A1 polypeptide) ELISA Kit; Coagulation factor XIII A chain ELISA Kit; Coagulation factor XIII A1 polypeptide ELISA Kit; Coagulation factor XIII A1 subunit ELISA Kit; Coagulation factor XIII; A polypeptide ELISA Kit; Coagulation factor XIIIa ELISA Kit; F13A ELISA Kit; F13A_HUMAN ELISA Kit; F13a1 ELISA Kit; Factor XIIIA ELISA Kit; Fibrin stabilizing factor; A subunit ELISA Kit; Fibrinoligase ELISA Kit; FSF; A subunit ELISA Kit; Protein glutamine gamma glutamyltransferase A chain ELISA Kit; Protein-glutamine gamma-glutamyltransferase A chain ELISA Kit; TGase ELISA Kit; Transglutaminase A chain ELISA Kit; Transglutaminase; plasma ELISA Kit; Transglutaminase. plasma ELISA Kit
缩写：F13A1
Uniprot No.：P00488
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Blood Coagulation
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human F13A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||
| Sample | Serum(n=4) | ||
| 1:1000 | Average % | 85 | |
| Range % | 81-89 | ||
| 1:2000 | Average % | 93 | |
| Range % | 90-96 | ||
| 1:4000 | Average % | 87 | |
| Range % | 83-90 | ||
| 1:8000 | Average % | 100 | |
| Range % | 97-103 |
回收率：
| The recovery of human F13A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 84 | 80-88 | ||||
| EDTA plasma (n=4) | 100 | 95-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 402.7832.7982.7912.622 202.3242.3682.3462.177 101.7681.7601.7641.595 51.1531.1681.1610.992 2.50.6910.6720.6820.513 1.250.4010.4060.4040.235 0.6250.2950.2760.2860.117 00.1700.1680.169 | ||||||
货期：3-5 working days
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