首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠凝血因子Ⅲ(FⅢ)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的F3含量。F3靶点是一种在癌症研究中具有重要意义的基因，其背景涉及多种癌症相关激酶靶点的构建和应用。研究机制方面，F3靶点与肿瘤细胞生长、凋亡、血管生成等密切相关，通过基因工程手段构建的Ba/F3细胞系可用于评估激酶抑制剂的靶向抑制作用。试剂盒检测范围为7.8 pg/mL-500 pg/mL，适用于小鼠疾病模型（如脓毒症、动脉粥样硬化等）中凝血功能研究、药物干预后FⅢ表达变化的检测，以及体外细胞实验中组织因子调控机制的探索，为心血管疾病和炎症相关研究的科研工作提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：F3; Cf-3; Cf3; Tissue factor; TF; Coagulation factor III; CD antigen CD142
缩写：F
Uniprot No.：P20352
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：7.8 pg/mL-500 pg/mL
灵敏度：1.95 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Blood Coagulation
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse CXCL3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 90-95 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 92-102 | |||||
| 1:4 | Average % | 103 | ||||
| Range % | 101-105 | |||||
| 1:8 | Average % | 89 | ||||
| Range % | 84-96 | |||||
回收率：
| The recovery of mouse CXCL3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 89-97 | ||||
| EDTA plasma (n=4) | 84 | 90-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected5002.8882.9952.9422.7832502.1022.2322.1672.0081251.3451.4321.3891.23062.50.8210.8330.8270.66831.20.5550.5100.5330.37415.60.3460.3310.3390.1807.80.2510.2690.2600.10100.1540.1630.159 | ||||||
货期：3-5 working days
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