首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠凝血因子Ⅶ(FⅦ)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的F7含量。小鼠凝血因子Ⅶ（FⅦ）是凝血系统中重要的因子，其基因剔除小鼠模型在研究凝血机制和抗血栓药物靶点方面具有重要作用。FⅦ基因剔除小鼠因缺乏FⅦ而表现出严重的出血倾向，为研究凝血因子功能提供了有力工具。试剂盒检测范围为0.45 ng/mL-30 ng/mL，该产品适用于凝血机制基础研究、抗凝药物开发评估以及血栓性疾病或出血性疾病小鼠模型中FⅦ蛋白水平的动态监测，为研究凝血调控网络提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：F7 ELISA Kit; Cf7 ELISA Kit; Coagulation factor VII ELISA Kit; EC 3.4.21.21 ELISA Kit; Serum prothrombin conversion accelerator) [Cleaved into: Factor VII light chain; Factor VII heavy chain] ELISA Kit
缩写：F7
Uniprot No.：P70375
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：0.45 ng/mL-30 ng/mL
灵敏度：0.11 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Blood Coagulation
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse FⅦ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1000 | Average % | 92 | |||||
| Range % | 86-96 | ||||||
| 1:2000 | Average % | 97 | |||||
| Range % | 92-102 | ||||||
| 1:4000 | Average % | 93 | |||||
| Range % | 88-97 | ||||||
| 1:8000 | Average % | 89 | |||||
| Range % | 84-94 | ||||||
回收率：
| The recovery of mouse FⅦ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 92 | 88-95 | |||||
| EDTA plasma (n=4) | 108 | 103-114 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected 302.4682.4452.4572.324 152.1151.9512.0331.900 7.51.5031.4401.4721.339 3.750.8450.7900.8180.685 1.80.6260.5930.6100.477 0.90.3810.3620.3720.239 0.450.2740.2520.2630.130 00.1370.1290.133 | |||||||
货期：3-5 working days
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