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Human coagulation factor VIII (FVⅢ) ELISA Kit

  • 中文名： 人凝血因子VIII(FVⅢ)ELISA Kit
货号: IPD-E13861h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人凝血因子VIII(FVⅢ)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的F8含量。试剂盒检测范围为12.5 ng/mL-800 ng/mL，该产品可应用于基础医学研究中凝血功能评估、血友病A动物模型机制探索、药物干预后FVIII水平变化监测等场景，为血液系统研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    AHF ELISA Kit; Antihemophilic factor ELISA Kit; Coagulation factor VIII ELISA Kit; coagulation factor VIII; procoagulant component ELISA Kit; coagulation factor VIIIc ELISA Kit; DXS1253E ELISA Kit; F8 ELISA Kit; F8b ELISA Kit; F8c ELISA Kit; FA8_HUMAN ELISA Kit; factor VIII F8B ELISA Kit; Factor VIIIa light chain ELISA Kit; FactorVIII ELISA Kit; FVIII ELISA Kit; Hema ELISA Kit; Hemophilia A ELISA Kit; Hemophilia; classic ELISA Kit; OTTHUMP00000061446 ELISA Kit; OTTHUMP00000196174 ELISA Kit; Procoagulant component ELISA Kit
  • 缩写：
    F8
  • Uniprot No.：
    P00451
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    12.5 ng/mL-800 ng/mL
  • 灵敏度：
    3.125 ng/ml.
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human FVⅢ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:100

    Average %

    106


    Range %

    99-112


    1:200

    Average %

    98


    Range %

    91-105


    1:400

    Average %

    104


    Range %

    99-110


    1:800

    Average %

    100


    Range %

    95-106


  • 回收率：

    The recovery of human FVⅢ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    96

    89-103


    EDTA plasma (n=4)

    101

    96-107


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    Corrected


    800

    2.734

    2.634

    2.684

    2.506


    400

    1.968

    1.867

    1.918

    1.740


    200

    1.462

    1.428

    1.445

    1.267


    100

    1.028

    1.019

    1.023

    0.846


    50

    0.642

    0.621

    0.632

    0.454


    25

    0.478

    0.453

    0.466

    0.288


    12.5

    0.319

    0.304

    0.312

    0.134


    0

    0.180

    0.175

    0.178



  • 本试剂盒所含材料：


    • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human FVIII antibody. This dismountable microplate can be divided into 12 x 8 strip plates.

    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.

    • One vial Biotin-labeled FVIII antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.

    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.

    • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.

    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.

    • 本试剂盒不含材料：


      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.

      • An incubator can provide stable incubation conditions up to 37°C±5°C.

      • Centrifuge

      • Vortex

      • Squirt bottle, manifold dispenser, or automated microplate washer

      • Absorbent paper for blotting the microtiter plate


      • 货期：
        3-5 working days

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