首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人脂肪酸酰胺水解酶1(FAAH)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FAAH含量。FAAH即脂肪酸酰胺水解酶，它能降解内源性大麻素等脂肪酸酰胺信号分子。其背景在于在调节机体疼痛、情绪等方面有重要作用。研究机制主要是通过对其活性的调控，影响内源性大麻素水平，进而探索治疗疼痛、焦虑等疾病的新策略。试剂盒检测范围为34.4 pg/mL-2200 pg/mL，适用于基础科研中探究FAAH在神经系统疾病、疼痛调控或代谢综合征中的表达变化，也可用于药物研发中酶活性抑制剂的体外筛选及作用机制分析，为神经生物学、药理学及分子病理学研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：FAAH; FAAH1; Fatty-acid amide hydrolase 1; Anandamide amidohydrolase 1; Fatty acid ester hydrolase; Oleamide hydrolase 1
缩写：FAAH
Uniprot No.：O00519
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：34.4 pg/mL-2200 pg/mL
灵敏度：8.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human FAAH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 91-101 | |||||
| 1:2 | Average % | 103 | ||||
| Range % | 99-107 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 87-97 | |||||
| 1:8 | Average % | 99 | ||||
| Range % | 91-102 | |||||
回收率：
| The recovery of human FAAH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 84-94 | ||||
| EDTA plasma (n=4) | 94 | 88-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected22002.4892.4132.4512.33211002.1172.0982.1081.9895501.4971.4521.4751.3562750.9480.9390.9440.825137.50.6220.6110.6170.49868.750.3750.3680.3720.25334.40.2880.2810.2850.16600.1210.1170.119 | ||||||
货期：3-5 working days
×
