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产品描述：人肝脏型脂肪酸结合蛋白(L-FABP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的FABP1含量。FABP1（脂肪酸结合蛋白1）是一种在肝脏中发现的脂肪酸结合蛋白，属于小型、高度保守的细胞质蛋白家族。其功能包括脂肪酸的摄取、运输和代谢。FABP1在肝脏细胞内脂质代谢中发挥重要作用，并可能参与细胞内脂质转运。研究显示，FABP1基因存在多种转录本和蛋白质变体，其突变可能导致疾病。试剂盒检测范围为31.2 pg/mL-2000pg/mL，适用于基础科研领域，如探究肝细胞模型中的脂代谢调控机制、评估药物或环境因素对肝脏功能的影响，或用于动物实验模型中肝脏损伤相关生物标志物的动态监测，为体外实验提供可靠的定量数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：FABP 1 ELISA Kit; FABP1 ELISA Kit; FABPL ELISA Kit; FABPL_HUMAN ELISA Kit; Fatty Acid Binding Protein 1 ELISA Kit; Fatty acid binding protein 1 liver ELISA Kit; Fatty Acid Binding Protein ELISA Kit; Fatty acid-binding protein 1 ELISA Kit; Fatty acid-binding protein ELISA Kit; Fatty acid-binding protein liver ELISA Kit; L FABP ELISA Kit; L-FABP ELISA Kit; liver ELISA Kit; Liver-type fatty acid-binding protein ELISA Kit
缩写：FABP1
Uniprot No.：P07148
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates
检测范围：31.2 pg/mL-2000pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human L-FABP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 86 | ||||
| Range % | 80-92 | |||||
| 1:200 | Average % | 98 | ||||
| Range % | 91-105 | |||||
| 1:400 | Average % | 98 | ||||
| Range % | 92-110 | |||||
| 1:800 | Average % | 94 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human L-FABP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 96 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.7162.6522.6842.60410001.6901.6761.6831.6035001.0491.0021.0260.9462500.5660.5390.5530.4731250.3420.3240.3330.25362.50.2080.1990.2040.12431.250.1480.1380.1430.06300.0830.0770.080 | ||||||
货期：3-5 working days
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